Improved protocols for histamine sensitization testing of acellular pertussis vaccines

Hokyung Oh; Jeewon Joung; Byoung Guk Kim; Kyung Tak Nam; Seung Hwa Hong; Hyun Chul Song; Ha Lim Lee; Byung Yoon Ahn

doi:10.1016/j.vaccine.2012.10.005

Improved protocols for histamine sensitization testing of acellular pertussis vaccines

Hokyung Oh, Jeewon Joung, Byoung Guk Kim, Kyung Tak Nam, Seung Hwa Hong, Hyun Chul Song, Ha Lim Lee, Byung Yoon Ahn

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The histamine sensitization test is a widely used method for measuring the residual toxicity of pertussis toxin in acellular pertussis vaccines. Although it has been used as a routine assay for decades, the current protocols are difficult to standardize because the test results vary considerably and are based on several factors, including mouse strain, age and sex. In this study, we observed that mice of strains CD1, ddY and C57/BL6 were sufficiently sensitive to pertussis toxin among six mice strains tested and that aged male mice were more sensitive to pertussis toxin than younger or female mice. Using this animal model, we showed pertussis toxin dose-dependent responses in the two histamine sensitization test protocols based on either lethal end-point determination or mouse rectal temperature measurement. Sensitivity to pertussis toxin was further enhanced by the addition of lipopolysaccharide in both methods. With these improvements, pertussis toxin activity can be estimated more accurately and reproducibly using a reduced number of animals.

Original language	English
Pages (from-to)	7246-7252
Number of pages	7
Journal	Vaccine
Volume	30
Issue number	50
DOIs	https://doi.org/10.1016/j.vaccine.2012.10.005
Publication status	Published - 2012 Nov 26

Bibliographical note

Funding Information:
This study was supported by a grant from the Scientific Research Program ( 11171KFDA353 ) at the National Institute of Food and Drug Safety Evaluation of the Korea Food and Drug Administration in the Republic of Korea .

Keywords

Acellular pertussis vaccine
HIST
Histamine sensitization test
LPS
Mouse model
Pertussis toxin

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2012.10.005

Cite this

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title = "Improved protocols for histamine sensitization testing of acellular pertussis vaccines",

abstract = "The histamine sensitization test is a widely used method for measuring the residual toxicity of pertussis toxin in acellular pertussis vaccines. Although it has been used as a routine assay for decades, the current protocols are difficult to standardize because the test results vary considerably and are based on several factors, including mouse strain, age and sex. In this study, we observed that mice of strains CD1, ddY and C57/BL6 were sufficiently sensitive to pertussis toxin among six mice strains tested and that aged male mice were more sensitive to pertussis toxin than younger or female mice. Using this animal model, we showed pertussis toxin dose-dependent responses in the two histamine sensitization test protocols based on either lethal end-point determination or mouse rectal temperature measurement. Sensitivity to pertussis toxin was further enhanced by the addition of lipopolysaccharide in both methods. With these improvements, pertussis toxin activity can be estimated more accurately and reproducibly using a reduced number of animals.",

keywords = "Acellular pertussis vaccine, HIST, Histamine sensitization test, LPS, Mouse model, Pertussis toxin",

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note = "Funding Information: This study was supported by a grant from the Scientific Research Program ( 11171KFDA353 ) at the National Institute of Food and Drug Safety Evaluation of the Korea Food and Drug Administration in the Republic of Korea .",

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AU - Hong, Seung Hwa

AU - Song, Hyun Chul

AU - Lee, Ha Lim

AU - Ahn, Byung Yoon

N1 - Funding Information: This study was supported by a grant from the Scientific Research Program ( 11171KFDA353 ) at the National Institute of Food and Drug Safety Evaluation of the Korea Food and Drug Administration in the Republic of Korea .

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N2 - The histamine sensitization test is a widely used method for measuring the residual toxicity of pertussis toxin in acellular pertussis vaccines. Although it has been used as a routine assay for decades, the current protocols are difficult to standardize because the test results vary considerably and are based on several factors, including mouse strain, age and sex. In this study, we observed that mice of strains CD1, ddY and C57/BL6 were sufficiently sensitive to pertussis toxin among six mice strains tested and that aged male mice were more sensitive to pertussis toxin than younger or female mice. Using this animal model, we showed pertussis toxin dose-dependent responses in the two histamine sensitization test protocols based on either lethal end-point determination or mouse rectal temperature measurement. Sensitivity to pertussis toxin was further enhanced by the addition of lipopolysaccharide in both methods. With these improvements, pertussis toxin activity can be estimated more accurately and reproducibly using a reduced number of animals.

AB - The histamine sensitization test is a widely used method for measuring the residual toxicity of pertussis toxin in acellular pertussis vaccines. Although it has been used as a routine assay for decades, the current protocols are difficult to standardize because the test results vary considerably and are based on several factors, including mouse strain, age and sex. In this study, we observed that mice of strains CD1, ddY and C57/BL6 were sufficiently sensitive to pertussis toxin among six mice strains tested and that aged male mice were more sensitive to pertussis toxin than younger or female mice. Using this animal model, we showed pertussis toxin dose-dependent responses in the two histamine sensitization test protocols based on either lethal end-point determination or mouse rectal temperature measurement. Sensitivity to pertussis toxin was further enhanced by the addition of lipopolysaccharide in both methods. With these improvements, pertussis toxin activity can be estimated more accurately and reproducibly using a reduced number of animals.

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Improved protocols for histamine sensitization testing of acellular pertussis vaccines

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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