Improvement of bioethanol production from waste chestnut shells via evaluation of mass balance-based pretreatment and glucose recovery process

Jeongho Lee, Seunghee Kim, Kang Hyun Lee, Soo Kweon Lee, Youngsang Chun, Seung Wook Kim, Chulhwan Park, Hah Young Yoo

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Chestnut shells (CSs) are a high-carbohydrate food waste generated by bioindustries. These are gaining popularity as promising biorefinery resources. In this study, we optimized the potassium hydroxide (KOH) pretreatment conditions for CS using response surface methodology in order to efficiently recover glucose via enzymatic saccharification. Here, biomass to glucose conversion (BtGC) was set as the response value, and the optimal conditions for maximum BtGC were determined using a prediction model in the following manner: CS loading, 92.7 g/L; temperature, 38.6 °C; time, 2.5 h. In the predictions and experiments, BtGC was 15.3% and 15.7% (glucan content: 69.1%, enzymatic digestibility [ED]: 39.7%, and solid recovery: 51.9%), respectively, a 2.5-fold improvement compared to the control group (6.3%). A profiling experiment was also performed to determine appropriate enzyme loading, resulting in an ED of 75.7%. Finally, CS hydrolysates were utilized as a medium for Saccharomyces cerevisiae K35 and had no inhibitory effect during fermentation. Finally, bioethanol production was estimated to be 150 g based on a mass balance of 1000 g CS. This study points in the right direction for achieving a sustainable society by valorizing food waste for biofuel production.

Original languageEnglish
Article number102955
JournalEnvironmental Technology and Innovation
Publication statusPublished - 2022 Nov

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT (MSIT) ( NRF-2020R1A2C1007493 and NRF-2020R1C1C1005060 ) and Kwangwoon University (2022). All authors have read and agreed to the published version of the manuscript.

Publisher Copyright:
© 2022 The Authors


  • Bioethanol fermentation
  • Chestnut shell
  • Enzymatic saccharification
  • Food processing waste
  • KOH pretreatment

ASJC Scopus subject areas

  • General Environmental Science
  • Soil Science
  • Plant Science


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