Abstract
Previously screened CoQ10-overproducing Agrobacterium tumefaciens A603-35 showed a relatively high NADH/NAD+ ratio (1.1), as compared to parental strain C58 (0.2) when we increased the expression levels of NADH-generating enzymes. Also, the intracellular NADH/NAD+ ratio showed a positive correlation with the CoQ10 content in A603-35. Overexpression of glyceraldehyde 3-phosphate dehydrogenase in A603-35 shifted the NADH/NAD+ ratio at 48 h from 0.8 to 1.2, and thus the CoQ 10 content in flask culture increased from 2.16 to 3.63 mg/g DCW. Due to the addition of hydroxybutyrate to the culture media, the intracellular NADH/NAD+ ratio in A603-35-gapA shifted from 1.2 to 1.4, which led to an increase CoQ10 content (5.27 mg/g DCW).
Original language | English |
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Pages (from-to) | 895-898 |
Number of pages | 4 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 74 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2010 |
Keywords
- Agrobacterium tumefaciens
- Coenzyme Q
- Fermentation
- Glyceraldehyde 3-phosphate dehydrogenase
- NADH/NAD ratio
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry