Improvement of enzymatic glucose conversion from chestnut shells through optimization of koh pretreatment

Kang Hyun Lee, Soo Kweon Lee, Jeongho Lee, Seunghee Kim, Chulhwan Park, Seung Wook Kim, Hah Young Yoo

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Worldwide, about one-third of food produced for human consumption is wasted, which includes byproducts from food processing, with a significant portion of the waste still being land-filled. The aim of this study is to convert chestnut shells (CNSs) from food processing into a valuable resource through bioprocesses. Currently, one of the highest barriers to bioprocess commercialization is low conversion of sugar from biomass, and KOH pretreatment was suggested to improve enzymatic digestibility (ED) of CNS. KOH concentration of 3% (w/w) was determined as a suitable pretreatment solution by a fundamental experiment. The reaction factors including temperature, time and solid/liquid (S/L) ratio were optimized (77.1 g/L CNS loading at 75 °C for 2.8 h) by response surface methodology (RSM). In the statistical model, temperature and time showed a relatively significant effect on the glucan content (GC) and ED, but S/L ratio was not. GC and ED of the untreated CNS were 45.1% and 12.7%, respectively. On the other hand, GC and ED of pretreated CNS were 83.2% and 48.4%, respectively, and which were significantly improved by about 1.8-fold and 3.8-fold compared to the control group. The improved ED through the optimization is expected to con-tribute to increasing the value of byproducts generated in food processing.

Original languageEnglish
Article number3772
JournalInternational journal of environmental research and public health
Issue number7
Publication statusPublished - 2021 Apr 1

Bibliographical note

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.


  • Chestnut shells
  • Enzymatic hydrolysis
  • Food processing wastes
  • KOH pretreatment
  • Statistical optimization

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis


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