Improvement of epothilone B production by in situ removal of ammonium using cation exchange resin in Sorangium cellulosum culture

Sang Woo Park; Se Jong Han; Dong Shik Kim; Sang Jun Sim

doi:10.1016/j.bej.2007.05.013

Improvement of epothilone B production by in situ removal of ammonium using cation exchange resin in Sorangium cellulosum culture

Sang Woo Park
, Se Jong Han
, Dong Shik Kim
, Sang Jun Sim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A cation exchange resin was added at different stages in the growth of Sorangium cellulosum cultures to remove excess ammonium, which has a negative influence on the production of the anticancer agent, epothilone. When 8 g L^-1 of cation exchange resin were added at the mid-death phase, the ammonium removal reached 83% and the maximum production of epothilone was 14.3 mg L^-1, which was 2.6 times greater than that of the control culture.

Original language	English
Pages (from-to)	328-331
Number of pages	4
Journal	Biochemical Engineering Journal
Volume	37
Issue number	3
DOIs	https://doi.org/10.1016/j.bej.2007.05.013
Publication status	Published - 2007 Dec 15
Externally published	Yes

Bibliographical note

Funding Information:
This research was supported by New Chemical Process Program under contact number 10023883 and Huons Co., Ltd. Korea.

Keywords

Ammonium
Cation resin
Epothilone
Feeding time
Sorangium cellulosum

ASJC Scopus subject areas

Biotechnology
Environmental Engineering
Bioengineering
Biomedical Engineering

Access to Document

10.1016/j.bej.2007.05.013

Cite this

@article{a882e6fc35d742e891ccbf2504c70499,

title = "Improvement of epothilone B production by in situ removal of ammonium using cation exchange resin in Sorangium cellulosum culture",

abstract = "A cation exchange resin was added at different stages in the growth of Sorangium cellulosum cultures to remove excess ammonium, which has a negative influence on the production of the anticancer agent, epothilone. When 8 g L-1 of cation exchange resin were added at the mid-death phase, the ammonium removal reached 83\% and the maximum production of epothilone was 14.3 mg L-1, which was 2.6 times greater than that of the control culture.",

keywords = "Ammonium, Cation resin, Epothilone, Feeding time, Sorangium cellulosum",

author = "Park, \{Sang Woo\} and Han, \{Se Jong\} and Kim, \{Dong Shik\} and Sim, \{Sang Jun\}",

note = "Funding Information: This research was supported by New Chemical Process Program under contact number 10023883 and Huons Co., Ltd. Korea.",

year = "2007",

month = dec,

day = "15",

doi = "10.1016/j.bej.2007.05.013",

language = "English",

volume = "37",

pages = "328--331",

journal = "Biochemical Engineering Journal",

issn = "1369-703X",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Improvement of epothilone B production by in situ removal of ammonium using cation exchange resin in Sorangium cellulosum culture

AU - Park, Sang Woo

AU - Han, Se Jong

AU - Kim, Dong Shik

AU - Sim, Sang Jun

N1 - Funding Information: This research was supported by New Chemical Process Program under contact number 10023883 and Huons Co., Ltd. Korea.

PY - 2007/12/15

Y1 - 2007/12/15

N2 - A cation exchange resin was added at different stages in the growth of Sorangium cellulosum cultures to remove excess ammonium, which has a negative influence on the production of the anticancer agent, epothilone. When 8 g L-1 of cation exchange resin were added at the mid-death phase, the ammonium removal reached 83% and the maximum production of epothilone was 14.3 mg L-1, which was 2.6 times greater than that of the control culture.

AB - A cation exchange resin was added at different stages in the growth of Sorangium cellulosum cultures to remove excess ammonium, which has a negative influence on the production of the anticancer agent, epothilone. When 8 g L-1 of cation exchange resin were added at the mid-death phase, the ammonium removal reached 83% and the maximum production of epothilone was 14.3 mg L-1, which was 2.6 times greater than that of the control culture.

KW - Ammonium

KW - Cation resin

KW - Epothilone

KW - Feeding time

KW - Sorangium cellulosum

UR - https://www.scopus.com/pages/publications/35548974646

U2 - 10.1016/j.bej.2007.05.013

DO - 10.1016/j.bej.2007.05.013

M3 - Article

AN - SCOPUS:35548974646

SN - 1369-703X

VL - 37

SP - 328

EP - 331

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

IS - 3

ER -

Improvement of epothilone B production by in situ removal of ammonium using cation exchange resin in Sorangium cellulosum culture

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this