In order to evaluate effects of γ-rays on adventitious root formation and ginsenoside production, embryogenic calli induced from cotyledon explants of Panax ginseng C. A. Meyer were treated with γ-rays of 0, 10, 30, 50, 70, and 100 Gy. The highest frequency of adventitious root formation of 75 % occurred at γ-irradiation of 30 Gy, which is considered adequate dosage for selecting mutant cell lines. Five mutated adventitious roots (MAR)3-lines out of the propagation of 142 adventitious root lines treated with 30 Gy were selected based a 100-fold increase in proliferation rate compared to control adventitious roots (CAR) and content of the seven major ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1) was determined. In the CAR and four of the MAR3-lines (except for MAR3-109), the Rb/Rg ratio was greater than 1.0, thereby indicating altered ginsenoside composition in these root lines. The HPLC analysis of the MAR3-13 and MAR3-26 lines confirmed different ginsenoside profiles, including the three unidentified ginsenoside candidates, Gm1, Gm2, and Gm3. The ginsenosides of the MAR3-13 and MAR3-26 lines showed high hydroxyl and superoxide radical scavenging activities.
Bibliographical noteFunding Information:
Received 14 June 2007, accepted 16 April 2008. Abbreviations: 2,4-D - 2,4 dichlorophenoxyacetic acid; CAR - control adventitious root; CL - chemiluminescence; IBA - indole-3-butyric acid; MAR - mutated adventitious root; MS - Murashige and Skoog; NAA - naphthaleneacetic acid. Acknowledgements: This work was conducted as a part of a project initiated by the Korea Atomic Energy Research Institute (KAERI), and was supported by the Korean Science and Engineering Foundation (KOSEF) in the Ministry of Education, Science and Technology (MEST). 1 Author for correspondence; fax: (+82) 63 570 3319; e-mail: email@example.com
- Radical scavenging activities
ASJC Scopus subject areas
- Plant Science