Improvement of ginsenoside production by Panax ginseng adventitious roots induced by γ-irradiation

D. S. Kim; S. Y. Kim; I. Y. Jeong; J. B. Kim; G. J. Lee; S. Y. Kang; W. Kim

doi:10.1007/s10535-009-0079-y

Improvement of ginsenoside production by Panax ginseng adventitious roots induced by γ-irradiation

D. S. Kim, S. Y. Kim, I. Y. Jeong, J. B. Kim, G. J. Lee, S. Y. Kang, W. Kim

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

In order to evaluate effects of γ-rays on adventitious root formation and ginsenoside production, embryogenic calli induced from cotyledon explants of Panax ginseng C. A. Meyer were treated with γ-rays of 0, 10, 30, 50, 70, and 100 Gy. The highest frequency of adventitious root formation of 75 % occurred at γ-irradiation of 30 Gy, which is considered adequate dosage for selecting mutant cell lines. Five mutated adventitious roots (MAR)3-lines out of the propagation of 142 adventitious root lines treated with 30 Gy were selected based a 100-fold increase in proliferation rate compared to control adventitious roots (CAR) and content of the seven major ginsenosides (Rb₁, Rb₂, Rc, Rd, Re, Rf, and Rg₁) was determined. In the CAR and four of the MAR3-lines (except for MAR3-109), the Rb/Rg ratio was greater than 1.0, thereby indicating altered ginsenoside composition in these root lines. The HPLC analysis of the MAR3-13 and MAR3-26 lines confirmed different ginsenoside profiles, including the three unidentified ginsenoside candidates, Gm₁, Gm₂, and Gm₃. The ginsenosides of the MAR3-13 and MAR3-26 lines showed high hydroxyl and superoxide radical scavenging activities.

Original language	English
Pages (from-to)	408-414
Number of pages	7
Journal	Biologia Plantarum
Volume	53
Issue number	3
DOIs	https://doi.org/10.1007/s10535-009-0079-y
Publication status	Published - 2009

Bibliographical note

Funding Information:
Received 14 June 2007, accepted 16 April 2008. Abbreviations: 2,4-D - 2,4 dichlorophenoxyacetic acid; CAR - control adventitious root; CL - chemiluminescence; IBA - indole-3-butyric acid; MAR - mutated adventitious root; MS - Murashige and Skoog; NAA - naphthaleneacetic acid. Acknowledgements: This work was conducted as a part of a project initiated by the Korea Atomic Energy Research Institute (KAERI), and was supported by the Korean Science and Engineering Foundation (KOSEF) in the Ministry of Education, Science and Technology (MEST). 1 Author for correspondence; fax: (+82) 63 570 3319; e-mail: bioplant@kaeri.re.kr

Keywords

HPLC
Mutation
Radical scavenging activities

ASJC Scopus subject areas

Plant Science
Horticulture

Access to Document

10.1007/s10535-009-0079-y

Cite this

@article{65c32fe3acbe40289bc15f1f51d39392,

title = "Improvement of ginsenoside production by Panax ginseng adventitious roots induced by γ-irradiation",

abstract = "In order to evaluate effects of γ-rays on adventitious root formation and ginsenoside production, embryogenic calli induced from cotyledon explants of Panax ginseng C. A. Meyer were treated with γ-rays of 0, 10, 30, 50, 70, and 100 Gy. The highest frequency of adventitious root formation of 75 % occurred at γ-irradiation of 30 Gy, which is considered adequate dosage for selecting mutant cell lines. Five mutated adventitious roots (MAR)3-lines out of the propagation of 142 adventitious root lines treated with 30 Gy were selected based a 100-fold increase in proliferation rate compared to control adventitious roots (CAR) and content of the seven major ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1) was determined. In the CAR and four of the MAR3-lines (except for MAR3-109), the Rb/Rg ratio was greater than 1.0, thereby indicating altered ginsenoside composition in these root lines. The HPLC analysis of the MAR3-13 and MAR3-26 lines confirmed different ginsenoside profiles, including the three unidentified ginsenoside candidates, Gm1, Gm2, and Gm3. The ginsenosides of the MAR3-13 and MAR3-26 lines showed high hydroxyl and superoxide radical scavenging activities.",

keywords = "HPLC, Mutation, Radical scavenging activities",

author = "Kim, {D. S.} and Kim, {S. Y.} and Jeong, {I. Y.} and Kim, {J. B.} and Lee, {G. J.} and Kang, {S. Y.} and W. Kim",

note = "Funding Information: Received 14 June 2007, accepted 16 April 2008. Abbreviations: 2,4-D - 2,4 dichlorophenoxyacetic acid; CAR - control adventitious root; CL - chemiluminescence; IBA - indole-3-butyric acid; MAR - mutated adventitious root; MS - Murashige and Skoog; NAA - naphthaleneacetic acid. Acknowledgements: This work was conducted as a part of a project initiated by the Korea Atomic Energy Research Institute (KAERI), and was supported by the Korean Science and Engineering Foundation (KOSEF) in the Ministry of Education, Science and Technology (MEST). 1 Author for correspondence; fax: (+82) 63 570 3319; e-mail: bioplant@kaeri.re.kr",

year = "2009",

doi = "10.1007/s10535-009-0079-y",

language = "English",

volume = "53",

pages = "408--414",

journal = "Biologia Plantarum",

issn = "0006-3134",

publisher = "Springer Netherlands",

number = "3",

}

TY - JOUR

T1 - Improvement of ginsenoside production by Panax ginseng adventitious roots induced by γ-irradiation

AU - Kim, D. S.

AU - Kim, S. Y.

AU - Jeong, I. Y.

AU - Kim, J. B.

AU - Lee, G. J.

AU - Kang, S. Y.

AU - Kim, W.

N1 - Funding Information: Received 14 June 2007, accepted 16 April 2008. Abbreviations: 2,4-D - 2,4 dichlorophenoxyacetic acid; CAR - control adventitious root; CL - chemiluminescence; IBA - indole-3-butyric acid; MAR - mutated adventitious root; MS - Murashige and Skoog; NAA - naphthaleneacetic acid. Acknowledgements: This work was conducted as a part of a project initiated by the Korea Atomic Energy Research Institute (KAERI), and was supported by the Korean Science and Engineering Foundation (KOSEF) in the Ministry of Education, Science and Technology (MEST). 1 Author for correspondence; fax: (+82) 63 570 3319; e-mail: bioplant@kaeri.re.kr

PY - 2009

Y1 - 2009

N2 - In order to evaluate effects of γ-rays on adventitious root formation and ginsenoside production, embryogenic calli induced from cotyledon explants of Panax ginseng C. A. Meyer were treated with γ-rays of 0, 10, 30, 50, 70, and 100 Gy. The highest frequency of adventitious root formation of 75 % occurred at γ-irradiation of 30 Gy, which is considered adequate dosage for selecting mutant cell lines. Five mutated adventitious roots (MAR)3-lines out of the propagation of 142 adventitious root lines treated with 30 Gy were selected based a 100-fold increase in proliferation rate compared to control adventitious roots (CAR) and content of the seven major ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1) was determined. In the CAR and four of the MAR3-lines (except for MAR3-109), the Rb/Rg ratio was greater than 1.0, thereby indicating altered ginsenoside composition in these root lines. The HPLC analysis of the MAR3-13 and MAR3-26 lines confirmed different ginsenoside profiles, including the three unidentified ginsenoside candidates, Gm1, Gm2, and Gm3. The ginsenosides of the MAR3-13 and MAR3-26 lines showed high hydroxyl and superoxide radical scavenging activities.

AB - In order to evaluate effects of γ-rays on adventitious root formation and ginsenoside production, embryogenic calli induced from cotyledon explants of Panax ginseng C. A. Meyer were treated with γ-rays of 0, 10, 30, 50, 70, and 100 Gy. The highest frequency of adventitious root formation of 75 % occurred at γ-irradiation of 30 Gy, which is considered adequate dosage for selecting mutant cell lines. Five mutated adventitious roots (MAR)3-lines out of the propagation of 142 adventitious root lines treated with 30 Gy were selected based a 100-fold increase in proliferation rate compared to control adventitious roots (CAR) and content of the seven major ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1) was determined. In the CAR and four of the MAR3-lines (except for MAR3-109), the Rb/Rg ratio was greater than 1.0, thereby indicating altered ginsenoside composition in these root lines. The HPLC analysis of the MAR3-13 and MAR3-26 lines confirmed different ginsenoside profiles, including the three unidentified ginsenoside candidates, Gm1, Gm2, and Gm3. The ginsenosides of the MAR3-13 and MAR3-26 lines showed high hydroxyl and superoxide radical scavenging activities.

KW - HPLC

KW - Mutation

KW - Radical scavenging activities

UR - http://www.scopus.com/inward/record.url?scp=70549096298&partnerID=8YFLogxK

U2 - 10.1007/s10535-009-0079-y

DO - 10.1007/s10535-009-0079-y

M3 - Article

AN - SCOPUS:70549096298

SN - 0006-3134

VL - 53

SP - 408

EP - 414

JO - Biologia Plantarum

JF - Biologia Plantarum

IS - 3

ER -

Improvement of ginsenoside production by Panax ginseng adventitious roots induced by γ-irradiation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this