Abstract
Inherent problems exist in the use of two-dimensional gel electrophoresis (2-DE) for sample preparation and separation of proteins from Halobacterium salinarum. In particular, proteins from cells grown in 25% NaCl are difficult to resolve by 2-DE due to the abundance of salt. To remove salts, a 3 kDa molecular weight cut-off column was used. When soluble proteins were separated by 2-DE, most of the proteins were concentrated in the acidic range. For separation of proteins in the pH 3-6 range, ultrazoom immobilized pH gradient strips were used. In addition, sample separation using a IPG-phor/Multiphor combined system was a more effective method for the proteome analysis of acidic proteins than using IPGphor for the isoelectric focusing step.
Original language | English |
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Pages (from-to) | 2325-2329 |
Number of pages | 5 |
Journal | Proteomics |
Volume | 3 |
Issue number | 12 |
DOIs | |
Publication status | Published - 2003 Dec |
Keywords
- Acidic protein
- Halobacterium salinarum
- IPGphor
- Multiphor combined system
- Two-dimensional gel electrophoresis
- Ultrazoom immobilized pH gradient strip
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology