Improving pneumovirus isolation using a centrifugation and AZD1480 combined method

Hansaem Lee; Hye Min Woo; Kisoon Kim; Sehee Park; Man Seong Park; Sung Soon Kim; You Jin Kim

doi:10.4014/jmb.1906.06050

Improving pneumovirus isolation using a centrifugation and AZD1480 combined method

Hansaem Lee, Hye Min Woo, Kisoon Kim, Sehee Park, Man Seong Park, Sung Soon Kim, You Jin Kim

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.

Original language	English
Pages (from-to)	2006-2013
Number of pages	8
Journal	Journal of microbiology and biotechnology
Volume	29
Issue number	12
DOIs	https://doi.org/10.4014/jmb.1906.06050
Publication status	Published - 2019

Bibliographical note

Funding Information:
We acknowledge Dr. Makoto Takeda for kindly providing the recombinant HMPV-GFP. This project was funded from the intramural research project no. 2015-NG47003-00, supported by the National Institute of Health, Korea Centers for Diseases Control and Prevention.

Funding Information:
This project was funded from the intramural research project no. 2015-NG47003-00, supported by the National I nstitute of Health, Korea Centers for Diseases Control and Prevention.

Publisher Copyright:
Copyright© 2019 by The Korean Society for Microbiology and Biotechnology.

Keywords

AZD1480
Centrifugation
Human metapneumovirus
Human respiratory syncytial virus
Virus isolation

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4014/jmb.1906.06050

Cite this

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title = "Improving pneumovirus isolation using a centrifugation and AZD1480 combined method",

abstract = "The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.",

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author = "Hansaem Lee and Woo, {Hye Min} and Kisoon Kim and Sehee Park and Park, {Man Seong} and Kim, {Sung Soon} and Kim, {You Jin}",

note = "Funding Information: We acknowledge Dr. Makoto Takeda for kindly providing the recombinant HMPV-GFP. This project was funded from the intramural research project no. 2015-NG47003-00, supported by the National Institute of Health, Korea Centers for Diseases Control and Prevention. Funding Information: This project was funded from the intramural research project no. 2015-NG47003-00, supported by the National I nstitute of Health, Korea Centers for Diseases Control and Prevention. Publisher Copyright: Copyright{\textcopyright} 2019 by The Korean Society for Microbiology and Biotechnology.",

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AU - Kim, Sung Soon

AU - Kim, You Jin

N1 - Funding Information: We acknowledge Dr. Makoto Takeda for kindly providing the recombinant HMPV-GFP. This project was funded from the intramural research project no. 2015-NG47003-00, supported by the National Institute of Health, Korea Centers for Diseases Control and Prevention. Funding Information: This project was funded from the intramural research project no. 2015-NG47003-00, supported by the National I nstitute of Health, Korea Centers for Diseases Control and Prevention. Publisher Copyright: Copyright© 2019 by The Korean Society for Microbiology and Biotechnology.

PY - 2019

Y1 - 2019

N2 - The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.

AB - The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.

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Improving pneumovirus isolation using a centrifugation and AZD1480 combined method

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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