The isolation of respiratory viruses, especially from clinical specimens, often shows poor efficiency with classical cell culture methods. The lack of suitable methods to generate virus particles inhibits the development of diagnostic assays, treatments, and vaccines. We compared three inoculation methods, classical cell culture, the addition of a JAK2 inhibitor AZD1480, and centrifugation-enhanced inoculation (CEI), to replicate human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV). In addition, a combined method using AZD1480 treatment and CEI was used on throat swabs to verify that this method could increase virus isolation efficiency from human clinical specimens. Both CEI and AZD1480 treatment increased HRSV and HMPV genome replication. Also, the combined method using CEI and AZD1480 treatment enhanced virus proliferation synergistically. The combined method is particularly suited for the isolation of interferon-sensitive or slowly growing viruses from human clinical specimens.
Bibliographical noteFunding Information:
We acknowledge Dr. Makoto Takeda for kindly providing the recombinant HMPV-GFP. This project was funded from the intramural research project no. 2015-NG47003-00, supported by the National Institute of Health, Korea Centers for Diseases Control and Prevention.
This project was funded from the intramural research project no. 2015-NG47003-00, supported by the National I nstitute of Health, Korea Centers for Diseases Control and Prevention.
Copyright© 2019 by The Korean Society for Microbiology and Biotechnology.
- Human metapneumovirus
- Human respiratory syncytial virus
- Virus isolation
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology