Improving quantitative analysis of phosphatidylcholine in lecithin using an HPLC-evaporative light-scattering detector

Jihye Moon, Su Hyun Chun, Kwang Won Lee

Research output: Contribution to journalArticlepeer-review

Abstract

The Health Functional Food Code uses a high-performance liquid chromatography (HPLC) refractive index detector (RID) to analyze phospholipids, the main ingredients in lecithin. However, RIDs have disadvantages of low sensitivity, high limits of detection (LOD) and quantification (LOQ), and complicated sample preparation. An evaporative light scattering detector (ELSD) with excellent sensitivity was used in this study. Validation showed high resolution, low LOD (22.64-32.57 μg/mL) and LOQ (68.60-98.68 μg/mL), and high reproducibility (98.15-104.29%). Uncertainties (95% confidence interval, k=2) were found to be 120.53±12.15 mg/g (soy lecithin) and 406.59±35.26 mg/g (egg yolk lecithin). The percent uncertainty of the measured concentrations (10.04% (soy lecithin) and 8.68% (egg yolk lecithin)) met the standards (11% or less) established by the European Union. Therefore, this HPLC-ELSD method shows excellent applicability for analyzing phosphatidylcholine in lecithin.

Original languageEnglish
Pages (from-to)294-302
Number of pages9
JournalKorean Journal of Food Science and Technology
Volume56
Issue number3
DOIs
Publication statusPublished - 2024 Jun

Bibliographical note

Publisher Copyright:
© 2024 The Korean Society of Food Science and Technology. All rights reserved.

Keywords

  • evaporative light-scattering detector
  • lecithin
  • phosphatidylcholine
  • uncertainty
  • validation

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

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