In-situ and highly sensitive detection of epidermal growth factor receptor mutation using nano-porous quartz crystal microbalance

Hyunjun Park; Juneseok You; Chanho Park; Kuewhan Jang; Sungsoo Na

doi:10.1007/s12206-018-0348-9

In-situ and highly sensitive detection of epidermal growth factor receptor mutation using nano-porous quartz crystal microbalance

Hyunjun Park, Juneseok You, Chanho Park, Kuewhan Jang, Sungsoo Na

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Assessment of epidermal growth factor receptor (EGFR) exon 19 mutations is very important indicator for treating lung cancer patients. In this report, we propose in-situ and highly sensitive detection method of EGFR mutation using quartz crystal microbalance (QCM) and nano-porous structured QCM electrode. The detection is based on the resonance frequency shift of QCM electrode upon DNA hybridization between probe DNA and EGFR mutant DNA (746–750 (del ELREA) in EGFR exon 19). Nano-porous structure is produced on the QCM electrode through simple processes of electrochemical deposition and etching. By using QCM, in-situ detection of EGFR mutation is performed within 30 mins. High sensitivity is achieved by introducing nano-porous structure on a QCM electrode, and the limit of detection (LOD) is 1 nM.

Original language	English
Pages (from-to)	1927-1932
Number of pages	6
Journal	Journal of Mechanical Science and Technology
Volume	32
Issue number	4
DOIs	https://doi.org/10.1007/s12206-018-0348-9
Publication status	Published - 2018 Apr 1

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) under Grant Number NRF-2014R1A2 A1A11052389, NRF-2016R1A5A1010148, NRF-2015M3A9 D7031026 and NRF-2016R1C1B1014817 which are funded by the Ministry of Science, ICT & Future Planning.

Publisher Copyright:
© 2018, The Korean Society of Mechanical Engineers and Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

Epidermal growth factor receptor (EGFR)
In-situ detection
Mutation
Nano-porous
Quartz crystal microbalance (QCM)

ASJC Scopus subject areas

Mechanics of Materials
Mechanical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12206-018-0348-9

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abstract = "Assessment of epidermal growth factor receptor (EGFR) exon 19 mutations is very important indicator for treating lung cancer patients. In this report, we propose in-situ and highly sensitive detection method of EGFR mutation using quartz crystal microbalance (QCM) and nano-porous structured QCM electrode. The detection is based on the resonance frequency shift of QCM electrode upon DNA hybridization between probe DNA and EGFR mutant DNA (746–750 (del ELREA) in EGFR exon 19). Nano-porous structure is produced on the QCM electrode through simple processes of electrochemical deposition and etching. By using QCM, in-situ detection of EGFR mutation is performed within 30 mins. High sensitivity is achieved by introducing nano-porous structure on a QCM electrode, and the limit of detection (LOD) is 1 nM.",

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author = "Hyunjun Park and Juneseok You and Chanho Park and Kuewhan Jang and Sungsoo Na",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) under Grant Number NRF-2014R1A2 A1A11052389, NRF-2016R1A5A1010148, NRF-2015M3A9 D7031026 and NRF-2016R1C1B1014817 which are funded by the Ministry of Science, ICT & Future Planning. Publisher Copyright: {\textcopyright} 2018, The Korean Society of Mechanical Engineers and Springer-Verlag GmbH Germany, part of Springer Nature.",

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AU - Jang, Kuewhan

AU - Na, Sungsoo

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) under Grant Number NRF-2014R1A2 A1A11052389, NRF-2016R1A5A1010148, NRF-2015M3A9 D7031026 and NRF-2016R1C1B1014817 which are funded by the Ministry of Science, ICT & Future Planning. Publisher Copyright: © 2018, The Korean Society of Mechanical Engineers and Springer-Verlag GmbH Germany, part of Springer Nature.

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Y1 - 2018/4/1

N2 - Assessment of epidermal growth factor receptor (EGFR) exon 19 mutations is very important indicator for treating lung cancer patients. In this report, we propose in-situ and highly sensitive detection method of EGFR mutation using quartz crystal microbalance (QCM) and nano-porous structured QCM electrode. The detection is based on the resonance frequency shift of QCM electrode upon DNA hybridization between probe DNA and EGFR mutant DNA (746–750 (del ELREA) in EGFR exon 19). Nano-porous structure is produced on the QCM electrode through simple processes of electrochemical deposition and etching. By using QCM, in-situ detection of EGFR mutation is performed within 30 mins. High sensitivity is achieved by introducing nano-porous structure on a QCM electrode, and the limit of detection (LOD) is 1 nM.

AB - Assessment of epidermal growth factor receptor (EGFR) exon 19 mutations is very important indicator for treating lung cancer patients. In this report, we propose in-situ and highly sensitive detection method of EGFR mutation using quartz crystal microbalance (QCM) and nano-porous structured QCM electrode. The detection is based on the resonance frequency shift of QCM electrode upon DNA hybridization between probe DNA and EGFR mutant DNA (746–750 (del ELREA) in EGFR exon 19). Nano-porous structure is produced on the QCM electrode through simple processes of electrochemical deposition and etching. By using QCM, in-situ detection of EGFR mutation is performed within 30 mins. High sensitivity is achieved by introducing nano-porous structure on a QCM electrode, and the limit of detection (LOD) is 1 nM.

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In-situ and highly sensitive detection of epidermal growth factor receptor mutation using nano-porous quartz crystal microbalance

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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