In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1α Peptide Eluting Vascular Grafts

Muhammad Shafiq, Qiuying Zhang, Dengke Zhi, Kai Wang, Deling Kong, Dong Hwee Kim, Soo Hyun Kim

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


Objective - The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell-derived factor)-1α-derived peptide and SP (substance P) for in situ vascular regeneration. Approach and Results - Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1α-derived peptide were fabricated by electrospinning. SP and SDF-1α peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1α and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1α grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood vessel formation, which was the most prominent in the SP grafts. Evaluation of inflammatory response showed that 3 groups did not significantly differ in terms of the numbers of proinflammatory macrophages, whereas SP grafts showed significantly higher numbers of proremodeling macrophages than that of the control and SDF-1α grafts. Conclusions - SDF-1α and SP grafts can be potential candidates for in situ vascular regeneration and are worthy for future investigations.

Original languageEnglish
Pages (from-to)E117-E134
JournalArteriosclerosis, thrombosis, and vascular biology
Issue number7
Publication statusPublished - 2018 Jul 1


  • endothelial cells
  • regeneration
  • stem cells
  • substance P
  • tissue engineering

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine


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