TY - JOUR
T1 - In situ chondrogenic differentiation of bone marrow stromal cells in bioactive self-assembled peptide gels
AU - Kim, Ji Eun
AU - Kim, Soo Hyun
AU - Jung, Youngmee
N1 - Funding Information:
This study was supported by the Korea Institute of Science and Technology Institutional Program ( 2E25119 and 2E24680 ).
Publisher Copyright:
© 2014 The Society for Biotechnology, Japan.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Articular cartilage is a specific tissue that lacks nerves and blood vessels and has limited self-repair abilities. Accordingly, it is necessary to develop new technology for the regeneration of cartilage to overcome therapeutic limitations. Recently, there have been several studies investigating the use of peptide hydrogel scaffolds, which are biocompatible and have low immunogenicity, for cartilage tissue engineering. In this study, we used self-assembled peptide hydrogels with repeating peptide sequences and bioactive motifs at the end of repeating sequences, which are collagen mimetic peptides (CMPs). CMPs that have a unique collagen-like triple helical conformation have been shown to associate with collagen molecules and fibers via a strand invasion process. In order to confirm the biological activities of the modified bioactive peptide hydrogels, the role of functional motifs in in situ chondrogenic differentiation of rabbit bone marrow stromal cells (rBMSCs) was examined. To compensate for the weaker mechanical properties of peptide hydrogels, we used poly (. l-lactide-co-caprolactone) (PLCL) scaffolds, which were loaded with the self-assembled peptides into which the bioactive motifs had been incorporated. Then, we performed in vitro and in vivo analyses with the rBMSC/PLCL-peptide hydrogel complexes. The results indicated that the secretion of a cartilage-specific extracellular matrix and gene expression concerned with chondrogenic differentiation were increased by CMP motifs. In conclusion, it was confirmed that CMP-modified self-assembled peptide hydrogels could effectively enhance chondrogenic differentiation in situ, and, consequently, they could be a good biomaterial for cartilage tissue engineering.
AB - Articular cartilage is a specific tissue that lacks nerves and blood vessels and has limited self-repair abilities. Accordingly, it is necessary to develop new technology for the regeneration of cartilage to overcome therapeutic limitations. Recently, there have been several studies investigating the use of peptide hydrogel scaffolds, which are biocompatible and have low immunogenicity, for cartilage tissue engineering. In this study, we used self-assembled peptide hydrogels with repeating peptide sequences and bioactive motifs at the end of repeating sequences, which are collagen mimetic peptides (CMPs). CMPs that have a unique collagen-like triple helical conformation have been shown to associate with collagen molecules and fibers via a strand invasion process. In order to confirm the biological activities of the modified bioactive peptide hydrogels, the role of functional motifs in in situ chondrogenic differentiation of rabbit bone marrow stromal cells (rBMSCs) was examined. To compensate for the weaker mechanical properties of peptide hydrogels, we used poly (. l-lactide-co-caprolactone) (PLCL) scaffolds, which were loaded with the self-assembled peptides into which the bioactive motifs had been incorporated. Then, we performed in vitro and in vivo analyses with the rBMSC/PLCL-peptide hydrogel complexes. The results indicated that the secretion of a cartilage-specific extracellular matrix and gene expression concerned with chondrogenic differentiation were increased by CMP motifs. In conclusion, it was confirmed that CMP-modified self-assembled peptide hydrogels could effectively enhance chondrogenic differentiation in situ, and, consequently, they could be a good biomaterial for cartilage tissue engineering.
KW - Bioactive
KW - Bone marrow stromal cells
KW - Collagen mimetic peptide
KW - In situ chondrogenic differentiation
KW - Self-assembled peptide
UR - http://www.scopus.com/inward/record.url?scp=84931564420&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2014.11.012
DO - 10.1016/j.jbiosc.2014.11.012
M3 - Article
C2 - 25540912
AN - SCOPUS:84931564420
SN - 1389-1723
VL - 120
SP - 91
EP - 98
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 1
ER -