In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering

Sang Soo Han, Hong Yeol Yoon, Ji Young Yhee, Myeong Ok Cho, Hye Eun Shim, Ji Eun Jeong, Dong Eun Lee, Kwangmeyung Kim, Hwanuk Guim, John Hwan Lee, Kang Moo Huh, Sun Woong Kang

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)


We report a biocompatible and in situ cross-linkable hydrogel derived from hyaluronic acid via a bioorthogonal reaction and confirm the clinical potential of our hydrogel through in vivo cartilage regeneration. Gelation is attributed to copper-free click reactions between an azide and dibenzyl cyclooctyne. HA-PEG4-DBCO was synthesized and cross-linked via 4-arm PEG azide. The effects of the ratio of HA-PEG4-DBCO to 4-arm PEG azide on the gelation time, microstructure, surface morphology, equilibrium swelling, and compressive modulus were examined. The potential of a hydrogel as an injectable scaffold was demonstrated by the encapsulation of chondrocytes within the hydrogel matrix in vitro and in vivo. The results demonstrated that the hydrogel supported cell survival, and the cells regenerated cartilaginous tissue. In addition, these characteristics provide potential opportunities for the use of injectable hydrogels in tissue engineering applications.

Original languageEnglish
Pages (from-to)20-27
Number of pages8
JournalPolymer Chemistry
Issue number1
Publication statusPublished - 2018 Jan 7
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by the INNOPOLIS Foundation grant (2016-02-DD-016), the Bio & Medical Technology Development Program of the National Research Foundation (NRF-2016 M3A9B4919616) funded by the Korean government (Ministry of Science, ICT & Future Planning). We thank Eun Hee Han of the Korea Basic Science Institute (Daejeon, Republic of Korea) for her technical support in the confocal microscopy analysis.

Publisher Copyright:
© The Royal Society of Chemistry 2018.

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Polymers and Plastics
  • Organic Chemistry


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