In situ detection of live cancer cells by using bioprobes based on Au nanoparticles

Jaemoon Yang; Kilho Eom; Eun Kyung Lim; Jinsung Park; Yoonah Kang; Dae Sung Yoon; Sungsoo Na; Eui Kwan Koh; Jin Suck Suh; Yong Min Huh; Tae Yun Kwon; Seungjoo Haam

doi:10.1021/la802184m

In situ detection of live cancer cells by using bioprobes based on Au nanoparticles

Jaemoon Yang, Kilho Eom, Eun Kyung Lim, Jinsung Park, Yoonah Kang, Dae Sung Yoon, Sungsoo Na, Eui Kwan Koh, Jin Suck Suh, Yong Min Huh, Tae Yun Kwon, Seungjoo Haam

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

We fabricate the high-performance probes based on Au nanoparticles (AuNP) for detection of live cancer cell. AuNP were synthesized with narrow sized distribution (ca. 10 nm) by Au salt reduction method and deposited onto the aminated substrate as a cross-linker and hot spot. Herein, AuNP has enabled the easy and efficient immobilization of the antibody (Cetuximab), which can selectively interact with epidermal growth factor receptor (EGFR) on the surface of epidermal cancer, as detecting moiety onto the AuNP-deposited substrate without nanolithography process. After conjugation of Cetuximab with AuNP-deposited substrate, Cetuximab-conjugated probe as a live cancer cell detector (LCCD) could detect EGFR-highexpressed A431 cells related to epithelial cancer with 54-times larger specificity and sensitivity in comparison with EGFR-deficient MCF7 cells. This implies that AuNP-based probes demonstrate abundant potentials for detection and separation of small biomolecules, cells and other chemicals.

Original language	English
Pages (from-to)	12112-12115
Number of pages	4
Journal	Langmuir
Volume	24
Issue number	21
DOIs	https://doi.org/10.1021/la802184m
Publication status	Published - 2008 Nov 4

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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10.1021/la802184m

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title = "In situ detection of live cancer cells by using bioprobes based on Au nanoparticles",

abstract = "We fabricate the high-performance probes based on Au nanoparticles (AuNP) for detection of live cancer cell. AuNP were synthesized with narrow sized distribution (ca. 10 nm) by Au salt reduction method and deposited onto the aminated substrate as a cross-linker and hot spot. Herein, AuNP has enabled the easy and efficient immobilization of the antibody (Cetuximab), which can selectively interact with epidermal growth factor receptor (EGFR) on the surface of epidermal cancer, as detecting moiety onto the AuNP-deposited substrate without nanolithography process. After conjugation of Cetuximab with AuNP-deposited substrate, Cetuximab-conjugated probe as a live cancer cell detector (LCCD) could detect EGFR-highexpressed A431 cells related to epithelial cancer with 54-times larger specificity and sensitivity in comparison with EGFR-deficient MCF7 cells. This implies that AuNP-based probes demonstrate abundant potentials for detection and separation of small biomolecules, cells and other chemicals.",

author = "Jaemoon Yang and Kilho Eom and Lim, {Eun Kyung} and Jinsung Park and Yoonah Kang and Yoon, {Dae Sung} and Sungsoo Na and Koh, {Eui Kwan} and Suh, {Jin Suck} and Huh, {Yong Min} and Kwon, {Tae Yun} and Seungjoo Haam",

year = "2008",

month = nov,

day = "4",

doi = "10.1021/la802184m",

language = "English",

volume = "24",

pages = "12112--12115",

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publisher = "American Chemical Society",

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TY - JOUR

T1 - In situ detection of live cancer cells by using bioprobes based on Au nanoparticles

AU - Yang, Jaemoon

AU - Eom, Kilho

AU - Lim, Eun Kyung

AU - Park, Jinsung

AU - Kang, Yoonah

AU - Yoon, Dae Sung

AU - Na, Sungsoo

AU - Koh, Eui Kwan

AU - Suh, Jin Suck

AU - Huh, Yong Min

AU - Kwon, Tae Yun

AU - Haam, Seungjoo

PY - 2008/11/4

Y1 - 2008/11/4

N2 - We fabricate the high-performance probes based on Au nanoparticles (AuNP) for detection of live cancer cell. AuNP were synthesized with narrow sized distribution (ca. 10 nm) by Au salt reduction method and deposited onto the aminated substrate as a cross-linker and hot spot. Herein, AuNP has enabled the easy and efficient immobilization of the antibody (Cetuximab), which can selectively interact with epidermal growth factor receptor (EGFR) on the surface of epidermal cancer, as detecting moiety onto the AuNP-deposited substrate without nanolithography process. After conjugation of Cetuximab with AuNP-deposited substrate, Cetuximab-conjugated probe as a live cancer cell detector (LCCD) could detect EGFR-highexpressed A431 cells related to epithelial cancer with 54-times larger specificity and sensitivity in comparison with EGFR-deficient MCF7 cells. This implies that AuNP-based probes demonstrate abundant potentials for detection and separation of small biomolecules, cells and other chemicals.

AB - We fabricate the high-performance probes based on Au nanoparticles (AuNP) for detection of live cancer cell. AuNP were synthesized with narrow sized distribution (ca. 10 nm) by Au salt reduction method and deposited onto the aminated substrate as a cross-linker and hot spot. Herein, AuNP has enabled the easy and efficient immobilization of the antibody (Cetuximab), which can selectively interact with epidermal growth factor receptor (EGFR) on the surface of epidermal cancer, as detecting moiety onto the AuNP-deposited substrate without nanolithography process. After conjugation of Cetuximab with AuNP-deposited substrate, Cetuximab-conjugated probe as a live cancer cell detector (LCCD) could detect EGFR-highexpressed A431 cells related to epithelial cancer with 54-times larger specificity and sensitivity in comparison with EGFR-deficient MCF7 cells. This implies that AuNP-based probes demonstrate abundant potentials for detection and separation of small biomolecules, cells and other chemicals.

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DO - 10.1021/la802184m

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AN - SCOPUS:56449128288

SN - 0743-7463

VL - 24

SP - 12112

EP - 12115

JO - Langmuir

JF - Langmuir

IS - 21

ER -

In situ detection of live cancer cells by using bioprobes based on Au nanoparticles

Abstract

ASJC Scopus subject areas

UN SDGs

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