In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells

Chandra Khatua; Sunhong Min; Hee Joon Jung; Hee Joon Jung; Hee Joon Jung; Jeong Eun Shin; Na Li; Indong Jun; Hui Wen Liu; Gunhyu Bae; Hyojun Choi; Min Jun Ko; Yoo Sang Jeon; Yu Jin Kim; Joonbum Lee; Minji Ko; Gyubo Shim; Hongchul Shin; Sangbum Lee; Seok Chung; Young Keun Kim; Jae Jun Song; Vinayak P. Dravid; Vinayak P. Dravid; Vinayak P. Dravid; Heemin Kang

doi:10.1021/acs.nanolett.0c00559

In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells

Chandra Khatua, Sunhong Min, Hee Joon Jung, Hee Joon Jung, Hee Joon Jung, Jeong Eun Shin, Na Li, Indong Jun, Hui Wen Liu, Gunhyu Bae, Hyojun Choi, Min Jun Ko, Yoo Sang Jeon, Yu Jin Kim, Joonbum Lee, Minji Ko, Gyubo Shim, Hongchul Shin, Sangbum Lee, Seok ChungYoung Keun Kim, Jae Jun Song, Vinayak P. Dravid, Vinayak P. Dravid, Vinayak P. Dravid, Heemin Kang

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Developing materials with remote controllability of macroscale ligand presentation can mimic extracellular matrix (ECM) remodeling to regulate cellular adhesion in vivo. Herein, we designed charged mobile nanoligands with superparamagnetic nanomaterials amine-functionalized and conjugated with polyethylene glycol linker and negatively charged RGD ligand. We coupled negatively a charged nanoligand to a positively charged substrate by optimizing electrostatic interactions to allow reversible planar movement. We demonstrate the imaging of both macroscale and in situ nanoscale nanoligand movement by magnetically attracting charged nanoligand to manipulate macroscale ligand density. We show that in situ magnetic control of attracting charged nanoligand facilitates stem cell adhesion, both in vitro and in vivo, with reversible control. Furthermore, we unravel that in situ magnetic attraction of charged nanoligand stimulates mechanosensing-mediated differentiation of stem cells. This remote controllability of ECM-mimicking reversible ligand variations is promising for regulating diverse reparative cellular processes in vivo.

Original language	English
Pages (from-to)	4188-4196
Number of pages	9
Journal	Nano Letters
Volume	20
Issue number	6
DOIs	https://doi.org/10.1021/acs.nanolett.0c00559
Publication status	Published - 2020 Jun 10

Bibliographical note

Publisher Copyright:
Copyright © 2020 American Chemical Society.

Keywords

cell adhesion
charged nanoligand
reversible ligand movement
spatial ligand movement
stem cell differentiation

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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Khatua, C., Min, S., Jung, H. J., Jung, H. J., Jung, H. J., Shin, J. E., Li, N., Jun, I., Liu, H. W., Bae, G., Choi, H., Ko, M. J., Jeon, Y. S., Kim, Y. J., Lee, J., Ko, M., Shim, G., Shin, H., Lee, S., ... Kang, H. (2020). In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells. Nano Letters, 20(6), 4188-4196. https://doi.org/10.1021/acs.nanolett.0c00559

Khatua, C, Min, S, Jung, HJ, Jung, HJ, Jung, HJ, Shin, JE, Li, N, Jun, I, Liu, HW, Bae, G, Choi, H, Ko, MJ, Jeon, YS, Kim, YJ, Lee, J, Ko, M, Shim, G, Shin, H, Lee, S, Chung, S , Kim, YK, Song, JJ, Dravid, VP, Dravid, VP, Dravid, VP & Kang, H 2020, 'In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells', Nano Letters, vol. 20, no. 6, pp. 4188-4196. https://doi.org/10.1021/acs.nanolett.0c00559

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abstract = "Developing materials with remote controllability of macroscale ligand presentation can mimic extracellular matrix (ECM) remodeling to regulate cellular adhesion in vivo. Herein, we designed charged mobile nanoligands with superparamagnetic nanomaterials amine-functionalized and conjugated with polyethylene glycol linker and negatively charged RGD ligand. We coupled negatively a charged nanoligand to a positively charged substrate by optimizing electrostatic interactions to allow reversible planar movement. We demonstrate the imaging of both macroscale and in situ nanoscale nanoligand movement by magnetically attracting charged nanoligand to manipulate macroscale ligand density. We show that in situ magnetic control of attracting charged nanoligand facilitates stem cell adhesion, both in vitro and in vivo, with reversible control. Furthermore, we unravel that in situ magnetic attraction of charged nanoligand stimulates mechanosensing-mediated differentiation of stem cells. This remote controllability of ECM-mimicking reversible ligand variations is promising for regulating diverse reparative cellular processes in vivo.",

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AU - Jun, Indong

AU - Liu, Hui Wen

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AU - Choi, Hyojun

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AU - Jeon, Yoo Sang

AU - Kim, Yu Jin

AU - Lee, Joonbum

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AU - Shim, Gyubo

AU - Shin, Hongchul

AU - Lee, Sangbum

AU - Chung, Seok

AU - Kim, Young Keun

AU - Song, Jae Jun

AU - Dravid, Vinayak P.

AU - Kang, Heemin

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In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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