@article{f0a36d8f2d4e45c48e79c61bb9a601bc,
title = "In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells",
abstract = "Developing materials with remote controllability of macroscale ligand presentation can mimic extracellular matrix (ECM) remodeling to regulate cellular adhesion in vivo. Herein, we designed charged mobile nanoligands with superparamagnetic nanomaterials amine-functionalized and conjugated with polyethylene glycol linker and negatively charged RGD ligand. We coupled negatively a charged nanoligand to a positively charged substrate by optimizing electrostatic interactions to allow reversible planar movement. We demonstrate the imaging of both macroscale and in situ nanoscale nanoligand movement by magnetically attracting charged nanoligand to manipulate macroscale ligand density. We show that in situ magnetic control of attracting charged nanoligand facilitates stem cell adhesion, both in vitro and in vivo, with reversible control. Furthermore, we unravel that in situ magnetic attraction of charged nanoligand stimulates mechanosensing-mediated differentiation of stem cells. This remote controllability of ECM-mimicking reversible ligand variations is promising for regulating diverse reparative cellular processes in vivo.",
keywords = "cell adhesion, charged nanoligand, reversible ligand movement, spatial ligand movement, stem cell differentiation",
author = "Chandra Khatua and Sunhong Min and Jung, {Hee Joon} and Jung, {Hee Joon} and Jung, {Hee Joon} and Shin, {Jeong Eun} and Na Li and Indong Jun and Liu, {Hui Wen} and Gunhyu Bae and Hyojun Choi and Ko, {Min Jun} and Jeon, {Yoo Sang} and Kim, {Yu Jin} and Joonbum Lee and Minji Ko and Gyubo Shim and Hongchul Shin and Sangbum Lee and Seok Chung and Kim, {Young Keun} and Song, {Jae Jun} and Dravid, {Vinayak P.} and Dravid, {Vinayak P.} and Dravid, {Vinayak P.} and Heemin Kang",
note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2019R1F1A1058720, 2019R1A2C3006587). This work was also supported by a Korea University Grant. This work was also conducted at the Korea Basic Science Institute. This work made use of the EPIC facility of Northwestern University{\textquoteright}s NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205), the MRSEC IRG2 program (NSF DMR-1720139) at the Materials Research Center, the International Institute for Nanotechnology (IIN), the Keck Foundation, and the State of Illinois through the IIN. Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2019R1F1A1058720, 2019R1A2C3006587). This work was also supported by a Korea University Grant. This work was also conducted at the Korea Basic Science Institute. This work made use of the EPIC facility of Northwestern University{\textquoteright}s NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205), the MRSEC IRG2 program (NSF DMR-1720139) at the Materials Research Center, the International Institute for Nanotechnology (IIN), the Keck Foundation, and the State of Illinois, through the IIN. Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society.",
year = "2020",
month = jun,
day = "10",
doi = "10.1021/acs.nanolett.0c00559",
language = "English",
volume = "20",
pages = "4188--4196",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "6",
}