In vitro and in vivo inhibitory effects of gaseous chlorine dioxide against Diaporthe batatas isolated from stored Sweetpotato

Ye Ji Lee, Jin Ju Jeong, Hyunjung Jin, Wook Kim, Gyeong Dan Yu, Ki Deok Kim

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Chlorine dioxide (ClO2) can be used as an alternative disinfectant for controlling fungal contamination during postharvest storage. In this study, we tested the in vitro and in vivo inhibitory effects of gaseous ClO2 against Diaporthe batatas SP-d1, the causal agent of sweetpotato dry rot. In in vitro tests, spore suspensions of SP-d1 spread on acidified potato dextrose agar were treated with various ClO2 concentrations (1-20 ppm) for 0-60 min. Fungal growth was significantly inhibited at 1 ppm of ClO2 treatment for 30 min, and completely inhibited at 20 ppm. In in vivo tests, spore suspensions were drop-inoculated onto sweetpotato slices, followed by ClO2 treatment with different concentrations and durations. Lesion diameters were not significantly different between the tested ClO2 concentrations; however, lesion diameters significantly decreased upon increasing the exposure time. Similarly, fungal populations decreased at the tested ClO2 concentrations over time. However, the sliced tissue itself hardened after 60-min ClO2 treatments, especially at 20 ppm of ClO2. When sweetpotato roots were dip-inoculated in spore suspensions for 10 min prior to treatment with 20 and 40 ppm of ClO2 for 0-60 min, fungal populations decreased with increasing ClO2 concentrations. Taken together, these results showed that gaseous ClO2 could significantly inhibit D. batatas growth and dry rot development in sweetpotato. Overall, gaseous ClO2 could be used to control this fungal disease during the postharvest storage of sweetpotato.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalPlant Pathology Journal
Issue number1
Publication statusPublished - 2019 Feb

Bibliographical note

Funding Information:
This work was supported by a grant from the Rural Development Administration (PJ011332) in Korea. J.-J. Jeong was supported by the Global Ph.D. program through the National Research Foundation of Korea funded by the Ministry of Education (2015-034526) in Korea.

Publisher Copyright:
© The Korean Society of Plant Pathology.


  • Chlorine dioxide
  • Diaporthe batatas
  • Dry rot
  • Sweetpotato

ASJC Scopus subject areas

  • Agronomy and Crop Science


Dive into the research topics of 'In vitro and in vivo inhibitory effects of gaseous chlorine dioxide against Diaporthe batatas isolated from stored Sweetpotato'. Together they form a unique fingerprint.

Cite this