In vitro micropropagation of a Cucurbita interspecific hybrid cultivar - A root stock plant

S. Sarowar; H. Y. Oh; N. I. Hyung; B. W. Min; C. H. Harn; S. K. Yang; S. H. Ok; J. S. Shin

doi:10.1023/A:1025043130423

In vitro micropropagation of a Cucurbita interspecific hybrid cultivar - A root stock plant

S. Sarowar, H. Y. Oh, N. I. Hyung, B. W. Min, C. H. Harn, S. K. Yang, S. H. Ok, J. S. Shin

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

An efficient in vitro micropropagation protocol was developed for direct shoot growth of interspecific Cucurbita hybrid variety using shoot-tips of 5-day-old explants. The excised shoot-tips were cultured on Murashige and Skoog (MS) medium containing two plant growth regulators (6-benzyladenine and naphthaleneacetic acid) with various combinations and concentrations for the study of shoot induction. The best condition for shoot growth was with 3 mg l^-1 6-benzyladenine (BA) in MS medium. The shooting frequency was 84% and five shoots were obtained from each explant after 30 days of culture. Shoots (1-1.5 cm length) were rooted most effectively in 1 mg l^-1 indole-3 butyric acid (IBA)-supplemented MS medium. The highest root formation rate was 93% and all rooted shoots were transplanted into soil.

Original language	English
Pages (from-to)	179-182
Number of pages	4
Journal	Plant Cell, Tissue and Organ Culture
Volume	75
Issue number	2
DOIs	https://doi.org/10.1023/A:1025043130423
Publication status	Published - 2003 Nov 1

Keywords

Cucurbita species
Interspecific hybrid variety
Micropropagation
Shoot tip multiplication

ASJC Scopus subject areas

Horticulture

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10.1023/A:1025043130423

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title = "In vitro micropropagation of a Cucurbita interspecific hybrid cultivar - A root stock plant",

abstract = "An efficient in vitro micropropagation protocol was developed for direct shoot growth of interspecific Cucurbita hybrid variety using shoot-tips of 5-day-old explants. The excised shoot-tips were cultured on Murashige and Skoog (MS) medium containing two plant growth regulators (6-benzyladenine and naphthaleneacetic acid) with various combinations and concentrations for the study of shoot induction. The best condition for shoot growth was with 3 mg l-1 6-benzyladenine (BA) in MS medium. The shooting frequency was 84% and five shoots were obtained from each explant after 30 days of culture. Shoots (1-1.5 cm length) were rooted most effectively in 1 mg l-1 indole-3 butyric acid (IBA)-supplemented MS medium. The highest root formation rate was 93% and all rooted shoots were transplanted into soil.",

keywords = "Cucurbita species, Interspecific hybrid variety, Micropropagation, Shoot tip multiplication",

author = "S. Sarowar and Oh, {H. Y.} and Hyung, {N. I.} and Min, {B. W.} and Harn, {C. H.} and Yang, {S. K.} and Ok, {S. H.} and Shin, {J. S.}",

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AU - Sarowar, S.

AU - Oh, H. Y.

AU - Hyung, N. I.

AU - Min, B. W.

AU - Harn, C. H.

AU - Yang, S. K.

AU - Ok, S. H.

AU - Shin, J. S.

PY - 2003/11/1

Y1 - 2003/11/1

N2 - An efficient in vitro micropropagation protocol was developed for direct shoot growth of interspecific Cucurbita hybrid variety using shoot-tips of 5-day-old explants. The excised shoot-tips were cultured on Murashige and Skoog (MS) medium containing two plant growth regulators (6-benzyladenine and naphthaleneacetic acid) with various combinations and concentrations for the study of shoot induction. The best condition for shoot growth was with 3 mg l-1 6-benzyladenine (BA) in MS medium. The shooting frequency was 84% and five shoots were obtained from each explant after 30 days of culture. Shoots (1-1.5 cm length) were rooted most effectively in 1 mg l-1 indole-3 butyric acid (IBA)-supplemented MS medium. The highest root formation rate was 93% and all rooted shoots were transplanted into soil.

AB - An efficient in vitro micropropagation protocol was developed for direct shoot growth of interspecific Cucurbita hybrid variety using shoot-tips of 5-day-old explants. The excised shoot-tips were cultured on Murashige and Skoog (MS) medium containing two plant growth regulators (6-benzyladenine and naphthaleneacetic acid) with various combinations and concentrations for the study of shoot induction. The best condition for shoot growth was with 3 mg l-1 6-benzyladenine (BA) in MS medium. The shooting frequency was 84% and five shoots were obtained from each explant after 30 days of culture. Shoots (1-1.5 cm length) were rooted most effectively in 1 mg l-1 indole-3 butyric acid (IBA)-supplemented MS medium. The highest root formation rate was 93% and all rooted shoots were transplanted into soil.

KW - Cucurbita species

KW - Interspecific hybrid variety

KW - Micropropagation

KW - Shoot tip multiplication

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ER -

In vitro micropropagation of a Cucurbita interspecific hybrid cultivar - A root stock plant

Abstract

Keywords

ASJC Scopus subject areas

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