In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines

Jinlong Yin, Xun Jin, Samuel Beck, Dong Ho Kang, Zhongshan Hong, Zhehu Li, Yongcheng Jin, Qiankun Zhang, Yun Jaie Choi, Sung Chan Kim, Hyunggee Kim

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Our current understanding of muscle and adipose tissue development has been largely restricted to the study of murine myogenic and adipogenic cell lines, since attempts to establish these cell lines from other species have met with only limited success. Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPARγ2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPARγ2-overexpressing BEFS cells (BEFS-PPARγ2) grown in ADCM with or without the PPARγ2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPARγ2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPARγ2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalBiotechnology letters
Issue number2
Publication statusPublished - 2010 Jan

Bibliographical note

Funding Information:
Acknowledgments This work was supported by grants (Code#20080401034047 and #20050301034441) from Bio-Green 21 Program, Rural Development Administration, Republic of Korea (to S.-C. Kim and H. Kim), and by Hallym University Research Fund, 2007 (HRF-2007-042; to S.-C. Kim).


  • Adipogenesis
  • BEFS-MyoD
  • Bovine embryonic fibroblast cell line
  • Myogenesis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines'. Together they form a unique fingerprint.

Cite this