In vitro nasal mucosa gland-like structure formation on a chip

Kyuhwan Na, Mingyu Lee, Hyun Woo Shin, Seok Chung

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

The emergence of microfluidic epithelial models using diverse types of cells within a physiologically relevant microenvironment has the potential to be a powerful tool for preclinical drug screening and pathophysiological studies. However, to date, few studies have reported the development of a complicated in vitro human nasal epithelial model. The aim of this study was to produce an in vitro human nasal mucosa model for reliable drug screening and clinical applications. Here, we integrated and optimized several culture conditions such as cell type, airway culture conditions, and hydrogel scaffolds into a microfluidic chip to construct an advanced in vitro human nasal mucosa model. We observed that the inducing factors for nasal gland-like structures were secreted from activated human dermal microvascular endothelial cells. Furthermore, our in vitro nasal mucosa presented different appearance and characteristics under hypoxic conditions. Morphological and functional similarities between in vivo nasal mucosa and our model indicated its utilization as a reliable research model for nasal diseases including allergic rhinitis, chronic sinusitis, and nasal polyposis.

Original languageEnglish
Pages (from-to)1578-1584
Number of pages7
JournalLab on a Chip
Volume17
Issue number9
DOIs
Publication statusPublished - 2017 May 7

Bibliographical note

Funding Information:
This work was supported by grant no. 03-2014-0120 from the SNUH Research Fund, by Creative-Pioneering Researchers Program through Seoul National University (SNU), by the Technological Innovation R&D Program (C0398093) funded by the Small and Medium Business Administration (SMBA, Korea), and by the National Research Foundation of Korea funded by the Ministry of Science (2014M3A7B4052193).

Publisher Copyright:
© The Royal Society of Chemistry 2017.

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • General Chemistry
  • Biomedical Engineering

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