In vitro photodynamic effects of scavenger receptor targeted-photoactivatable nanoagents on activated macrophages

Bong Gu Yi; Ok Kyu Park; Myeong Seon Jeong; Seung Hae Kwon; Jae In Jung; Seongsoo Lee; Sungwoo Ryoo; Sung Eun Kim; Jin Won Kim; Won Jin Moon; Kyeongsoon Park

doi:10.1016/j.ijbiomac.2017.01.037

In vitro photodynamic effects of scavenger receptor targeted-photoactivatable nanoagents on activated macrophages

Bong Gu Yi, Ok Kyu Park, Myeong Seon Jeong, Seung Hae Kwon, Jae In Jung, Seongsoo Lee, Sungwoo Ryoo, Sung Eun Kim, Jin Won Kim, Won Jin Moon, Kyeongsoon Park

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Scavenger receptors (SRs) expressed on the activated macrophages in inflammation sites have been considered as the most interesting and important target biomarker for targeted drug delivery, imaging and therapy. In the present study, we fabricated the scavenger receptor-A (SR-A) targeted-photoactivatable nanoagents (termed as Ce6/DS-DOCA) by entrapping chlorin e6 (Ce6) into the amphiphilic dextran sulfate-deoxycholic acid (DS-DOCA) conjugates via physically hydrophobic interactions. Insoluble Ce6 was easily encapsulated into DS-DOCA nanoparticles by a dialysis method and the loading efficiency was approximately 51.7%. The Ce6/DS-DOCA formed nano-sized self-assembled aggregates (28.8 ± 5.6 nm in diameter), confirmed by transmission electron microscope, UV/Vis and fluorescence spectrophotometer. The Ce6/DS-DOCA nanoagents could generate highly reactive singlet oxygen under laser irradiation. Also, in vitro studies showed that they were more specifically taken up by lipopolysaccharide (LPS)-induced activated macrophages (RAW 264.7) via a SR-A-mediated endocytosis, relative to by non-activated macrophages, and notably induced cell death of activated macrophages under laser irradiation. Therefore, SR-A targetable and photoactivatable Ce6/DS-DOCA nanoagents with more selective targeting to the activated macrophages will have great potential for treatment of inflammatory diseases.

Original language	English
Pages (from-to)	181-189
Number of pages	9
Journal	International Journal of Biological Macromolecules
Volume	97
DOIs	https://doi.org/10.1016/j.ijbiomac.2017.01.037
Publication status	Published - 2017 Apr 1
Externally published	Yes

Keywords

Activated macrophages
Photoactivatable nanoagents
Scavenger receptor targeting

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology
Economics and Econometrics
Energy(all)

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10.1016/j.ijbiomac.2017.01.037

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@article{b502d4a244a54467baff5b700834700c,

title = "In vitro photodynamic effects of scavenger receptor targeted-photoactivatable nanoagents on activated macrophages",

abstract = "Scavenger receptors (SRs) expressed on the activated macrophages in inflammation sites have been considered as the most interesting and important target biomarker for targeted drug delivery, imaging and therapy. In the present study, we fabricated the scavenger receptor-A (SR-A) targeted-photoactivatable nanoagents (termed as Ce6/DS-DOCA) by entrapping chlorin e6 (Ce6) into the amphiphilic dextran sulfate-deoxycholic acid (DS-DOCA) conjugates via physically hydrophobic interactions. Insoluble Ce6 was easily encapsulated into DS-DOCA nanoparticles by a dialysis method and the loading efficiency was approximately 51.7%. The Ce6/DS-DOCA formed nano-sized self-assembled aggregates (28.8 ± 5.6 nm in diameter), confirmed by transmission electron microscope, UV/Vis and fluorescence spectrophotometer. The Ce6/DS-DOCA nanoagents could generate highly reactive singlet oxygen under laser irradiation. Also, in vitro studies showed that they were more specifically taken up by lipopolysaccharide (LPS)-induced activated macrophages (RAW 264.7) via a SR-A-mediated endocytosis, relative to by non-activated macrophages, and notably induced cell death of activated macrophages under laser irradiation. Therefore, SR-A targetable and photoactivatable Ce6/DS-DOCA nanoagents with more selective targeting to the activated macrophages will have great potential for treatment of inflammatory diseases.",

keywords = "Activated macrophages, Photoactivatable nanoagents, Scavenger receptor targeting",

author = "Yi, {Bong Gu} and Park, {Ok Kyu} and Jeong, {Myeong Seon} and Kwon, {Seung Hae} and Jung, {Jae In} and Seongsoo Lee and Sungwoo Ryoo and Kim, {Sung Eun} and Kim, {Jin Won} and Moon, {Won Jin} and Kyeongsoon Park",

note = "Funding Information: This work received partial financial support from grants provided by KBSI (E36200), and the National Research Foundation of Korea (NRF), the Ministry of Education, Science and Technology (grant number 2015R1A2A2A07027863 and 2015R1D1A1A01059079). Publisher Copyright: {\textcopyright} 2017",

year = "2017",

month = apr,

day = "1",

doi = "10.1016/j.ijbiomac.2017.01.037",

language = "English",

volume = "97",

pages = "181--189",

journal = "International Journal of Biological Macromolecules",

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T1 - In vitro photodynamic effects of scavenger receptor targeted-photoactivatable nanoagents on activated macrophages

AU - Yi, Bong Gu

AU - Park, Ok Kyu

AU - Jeong, Myeong Seon

AU - Kwon, Seung Hae

AU - Jung, Jae In

AU - Lee, Seongsoo

AU - Ryoo, Sungwoo

AU - Kim, Sung Eun

AU - Kim, Jin Won

AU - Moon, Won Jin

AU - Park, Kyeongsoon

N1 - Funding Information: This work received partial financial support from grants provided by KBSI (E36200), and the National Research Foundation of Korea (NRF), the Ministry of Education, Science and Technology (grant number 2015R1A2A2A07027863 and 2015R1D1A1A01059079). Publisher Copyright: © 2017

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Scavenger receptors (SRs) expressed on the activated macrophages in inflammation sites have been considered as the most interesting and important target biomarker for targeted drug delivery, imaging and therapy. In the present study, we fabricated the scavenger receptor-A (SR-A) targeted-photoactivatable nanoagents (termed as Ce6/DS-DOCA) by entrapping chlorin e6 (Ce6) into the amphiphilic dextran sulfate-deoxycholic acid (DS-DOCA) conjugates via physically hydrophobic interactions. Insoluble Ce6 was easily encapsulated into DS-DOCA nanoparticles by a dialysis method and the loading efficiency was approximately 51.7%. The Ce6/DS-DOCA formed nano-sized self-assembled aggregates (28.8 ± 5.6 nm in diameter), confirmed by transmission electron microscope, UV/Vis and fluorescence spectrophotometer. The Ce6/DS-DOCA nanoagents could generate highly reactive singlet oxygen under laser irradiation. Also, in vitro studies showed that they were more specifically taken up by lipopolysaccharide (LPS)-induced activated macrophages (RAW 264.7) via a SR-A-mediated endocytosis, relative to by non-activated macrophages, and notably induced cell death of activated macrophages under laser irradiation. Therefore, SR-A targetable and photoactivatable Ce6/DS-DOCA nanoagents with more selective targeting to the activated macrophages will have great potential for treatment of inflammatory diseases.

AB - Scavenger receptors (SRs) expressed on the activated macrophages in inflammation sites have been considered as the most interesting and important target biomarker for targeted drug delivery, imaging and therapy. In the present study, we fabricated the scavenger receptor-A (SR-A) targeted-photoactivatable nanoagents (termed as Ce6/DS-DOCA) by entrapping chlorin e6 (Ce6) into the amphiphilic dextran sulfate-deoxycholic acid (DS-DOCA) conjugates via physically hydrophobic interactions. Insoluble Ce6 was easily encapsulated into DS-DOCA nanoparticles by a dialysis method and the loading efficiency was approximately 51.7%. The Ce6/DS-DOCA formed nano-sized self-assembled aggregates (28.8 ± 5.6 nm in diameter), confirmed by transmission electron microscope, UV/Vis and fluorescence spectrophotometer. The Ce6/DS-DOCA nanoagents could generate highly reactive singlet oxygen under laser irradiation. Also, in vitro studies showed that they were more specifically taken up by lipopolysaccharide (LPS)-induced activated macrophages (RAW 264.7) via a SR-A-mediated endocytosis, relative to by non-activated macrophages, and notably induced cell death of activated macrophages under laser irradiation. Therefore, SR-A targetable and photoactivatable Ce6/DS-DOCA nanoagents with more selective targeting to the activated macrophages will have great potential for treatment of inflammatory diseases.

KW - Activated macrophages

KW - Photoactivatable nanoagents

KW - Scavenger receptor targeting

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DO - 10.1016/j.ijbiomac.2017.01.037

M3 - Article

C2 - 28082222

AN - SCOPUS:85009136747

SN - 0141-8130

VL - 97

SP - 181

EP - 189

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

In vitro photodynamic effects of scavenger receptor targeted-photoactivatable nanoagents on activated macrophages

Abstract

Keywords

ASJC Scopus subject areas

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