In vitro trans-differentiation of rat mesenchymal cells into insulin-producing cells by rat pancreatic extract

Kyung Suk Choi; Jun Seop Shin; Jae Jeong Lee; Young Soo Kim; Seung Bum Kim; Chan Wha Kim

doi:10.1016/j.bbrc.2005.03.111

In vitro trans-differentiation of rat mesenchymal cells into insulin-producing cells by rat pancreatic extract

Kyung Suk Choi, Jun Seop Shin, Jae Jeong Lee, Young Soo Kim, Seung Bum Kim, Chan Wha Kim

Research output: Contribution to journal › Article › peer-review

185 Citations (Scopus)

Abstract

Recent reports have suggested that mesenchymal cells derived from bone marrow may differentiate into not only mesenchymal lineage cells but also other lineage cells. There is possibility for insulin-producing cells (IPCs) to be differentiated from mesenchymal cells. We used self-functional repair stimuli of stem cells by partial injury. Rat pancreatic extract (RPE) from the regenerating pancreas (2 days after 60% pancreatectomy) was treated to rat mesenchymal cells. After the treatment of RPE, they made clusters like islet of Langerhans within a week and expressed four pancreatic endocrine hormones; insulin, glucagon, pancreatic polypeptide, and somatostatin. Moreover, IPCs released insulin in response to normal glucose challenge. Here we demonstrate that the treatment of RPE can differentiate rat mesenchymal cells into IPCs which can be a potential source for the therapy of diabetes.

Original language	English
Pages (from-to)	1299-1305
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	330
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2005.03.111
Publication status	Published - 2005 May 20

Bibliographical note

Funding Information:
This study was supported by a grant from Korea Food and Drug Administration (03092-073). Kyung Suk Choi, Jun-Seop Shin, Jae-Jeong Lee, Young Soo Kim, and Seung-Bum Kim held a Brain Korea 21 fellowship from Korea Ministry of Education and Human Resources Development.

Keywords

Bone marrow stromal cell
Differentiation
Mesenchymal stem cell
Pancreatectomy

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2005.03.111

Cite this

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title = "In vitro trans-differentiation of rat mesenchymal cells into insulin-producing cells by rat pancreatic extract",

abstract = "Recent reports have suggested that mesenchymal cells derived from bone marrow may differentiate into not only mesenchymal lineage cells but also other lineage cells. There is possibility for insulin-producing cells (IPCs) to be differentiated from mesenchymal cells. We used self-functional repair stimuli of stem cells by partial injury. Rat pancreatic extract (RPE) from the regenerating pancreas (2 days after 60% pancreatectomy) was treated to rat mesenchymal cells. After the treatment of RPE, they made clusters like islet of Langerhans within a week and expressed four pancreatic endocrine hormones; insulin, glucagon, pancreatic polypeptide, and somatostatin. Moreover, IPCs released insulin in response to normal glucose challenge. Here we demonstrate that the treatment of RPE can differentiate rat mesenchymal cells into IPCs which can be a potential source for the therapy of diabetes.",

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note = "Funding Information: This study was supported by a grant from Korea Food and Drug Administration (03092-073). Kyung Suk Choi, Jun-Seop Shin, Jae-Jeong Lee, Young Soo Kim, and Seung-Bum Kim held a Brain Korea 21 fellowship from Korea Ministry of Education and Human Resources Development.",

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AU - Kim, Seung Bum

AU - Kim, Chan Wha

N1 - Funding Information: This study was supported by a grant from Korea Food and Drug Administration (03092-073). Kyung Suk Choi, Jun-Seop Shin, Jae-Jeong Lee, Young Soo Kim, and Seung-Bum Kim held a Brain Korea 21 fellowship from Korea Ministry of Education and Human Resources Development.

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AB - Recent reports have suggested that mesenchymal cells derived from bone marrow may differentiate into not only mesenchymal lineage cells but also other lineage cells. There is possibility for insulin-producing cells (IPCs) to be differentiated from mesenchymal cells. We used self-functional repair stimuli of stem cells by partial injury. Rat pancreatic extract (RPE) from the regenerating pancreas (2 days after 60% pancreatectomy) was treated to rat mesenchymal cells. After the treatment of RPE, they made clusters like islet of Langerhans within a week and expressed four pancreatic endocrine hormones; insulin, glucagon, pancreatic polypeptide, and somatostatin. Moreover, IPCs released insulin in response to normal glucose challenge. Here we demonstrate that the treatment of RPE can differentiate rat mesenchymal cells into IPCs which can be a potential source for the therapy of diabetes.

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In vitro trans-differentiation of rat mesenchymal cells into insulin-producing cells by rat pancreatic extract

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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