In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter

Boo Ja Lee; Chang Jin Park; Sung Kyu Kim; Ki Jeong Kim; Kyung Hee Paek

doi:10.1016/j.bbrc.2006.03.153

In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter

Boo Ja Lee, Chang Jin Park, Sung Kyu Kim, Ki Jeong Kim, Kyung Hee Paek

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.

Original language	English
Pages (from-to)	55-62
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	344
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2006.03.153
Publication status	Published - 2006 May 26

Bibliographical note

Funding Information:
This study was supported by a grant (R11-2003-008-02001-0) from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center.

Keywords

CaPR-1 promoter
CabZIP1
Chromatin immunoprecipitation (ChIP)
Hot pepper (Capsicum annuum L.)
Nuclear localization

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2006.03.153

Cite this

@article{a3171329708c43b180dfe7b3e7d2fdc6,

title = "In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter",

abstract = "We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.",

keywords = "CaPR-1 promoter, CabZIP1, Chromatin immunoprecipitation (ChIP), Hot pepper (Capsicum annuum L.), Nuclear localization",

author = "Lee, {Boo Ja} and Park, {Chang Jin} and Kim, {Sung Kyu} and Kim, {Ki Jeong} and Paek, {Kyung Hee}",

note = "Funding Information: This study was supported by a grant (R11-2003-008-02001-0) from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center.",

year = "2006",

month = may,

day = "26",

doi = "10.1016/j.bbrc.2006.03.153",

language = "English",

volume = "344",

pages = "55--62",

journal = "Biochemical and biophysical research communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter

AU - Lee, Boo Ja

AU - Park, Chang Jin

AU - Kim, Sung Kyu

AU - Kim, Ki Jeong

AU - Paek, Kyung Hee

N1 - Funding Information: This study was supported by a grant (R11-2003-008-02001-0) from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center.

PY - 2006/5/26

Y1 - 2006/5/26

N2 - We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.

AB - We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.

KW - CaPR-1 promoter

KW - CabZIP1

KW - Chromatin immunoprecipitation (ChIP)

KW - Hot pepper (Capsicum annuum L.)

KW - Nuclear localization

UR - http://www.scopus.com/inward/record.url?scp=33646486277&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.03.153

DO - 10.1016/j.bbrc.2006.03.153

M3 - Article

C2 - 16630556

AN - SCOPUS:33646486277

SN - 0006-291X

VL - 344

SP - 55

EP - 62

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

IS - 1

ER -

In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this