In vivo direct imaging of neuronal activity at high temporospatial resolution

Phan Tan Toi, Hyun Jae Jang, Kyeongseon Min, Sung Phil Kim, Seung Kyun Lee, Jongho Lee, Jeehyun Kwag, Jang Yeon Park

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


There has been a long-standing demand for noninvasive neuroimaging methods that can detect neuronal activity at both high temporal and high spatial resolution. We present a two-dimensional fast line-scan approach that enables direct imaging of neuronal activity with millisecond precision while retaining the high spatial resolution of magnetic resonance imaging (MRI). This approach was demonstrated through in vivo mouse brain imaging at 9.4 tesla during electrical whisker-pad stimulation. In vivo spike recording and optogenetics confirmed the high correlation of the observed MRI signal with neural activity. It also captured the sequential and laminar-specific propagation of neuronal activity along the thalamocortical pathway. This high-resolution, direct imaging of neuronal activity will open up new avenues in brain science by providing a deeper understanding of the brain’s functional organization, including the temporospatial dynamics of neural networks.

Original languageEnglish
Article number378
Issue number6616
Publication statusPublished - 2022 Oct 14

ASJC Scopus subject areas

  • General


Dive into the research topics of 'In vivo direct imaging of neuronal activity at high temporospatial resolution'. Together they form a unique fingerprint.

Cite this