In vivo imaging of endogenously produced HCLO in zebrafish and mice using a bright, photostable ratiometric fluorescent probe

Chong Duan; Miae Won; Peter Verwilst; Junchao Xu; Hyeong Seok Kim; Lintao Zeng; Jong Seung Kim

doi:10.1021/acs.analchem.9b00224

In vivo imaging of endogenously produced HCLO in zebrafish and mice using a bright, photostable ratiometric fluorescent probe

Chong Duan, Miae Won, Peter Verwilst, Junchao Xu, Hyeong Seok Kim, Lintao Zeng, Jong Seung Kim

Research output: Contribution to journal › Article › peer-review

297 Citations (Scopus)

Abstract

A high brightness red fluorescent probe (S-BODIPY) has been developed for the sensitive and specific imaging of HClO/ClO ^- in vitro and in vivo. This probe exhibits some distinctive features such as excellent resistance to photobleaching, a high fluorescence brightness, high selectivity, as well as a good biocompatibility. Upon oxidation of the thio-ether group into sulfoxide, the probe showed a noticeable ratiometric fluorescence response toward ClO ^- with fast response (within 30 s) and a low detection limit (59 nM). The probe demonstrated the successful imaging of exogenous and endogenous HClO/ClO ^- in living HeLa cells, zebrafish, and mice with high signal-to-noise ratios. S-BODIPY allows for the real-time monitoring the level of ClO ^- in living cells by ratiometric fluorescence imaging, opening up exciting prospects to develop red and even near-infrared BODIPYs with high brightness and good photostability for in vivo imaging.

Original language	English
Pages (from-to)	4172-4178
Number of pages	7
Journal	Analytical chemistry
Volume	91
Issue number	6
DOIs	https://doi.org/10.1021/acs.analchem.9b00224
Publication status	Published - 2019 Mar 19

Bibliographical note

Funding Information:
2018R1A3B1052702, J.S.K.), and the Basic Science Research Program (2017R1D1A1B03032561, P.V. and 2017R1D1A1B03030062, M.W.) funded by the Ministry of Education as well as the Korea Research Fellowship Program funded by the Ministry of Science and ICT through the National Research Foundation of Korea (2016H1D3A1938052, P.V.).

Funding Information:
We gratefully thank the financial support from the National Natural Science Foundation of China (No.21203138, L.Z.) and the Natural Science Foundation of Tianjin (17JCYBJC19600, L.Z.). This work was also supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (CRI project no.

Publisher Copyright:
© 2019 American Chemical Society.

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/acs.analchem.9b00224

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title = "In vivo imaging of endogenously produced HCLO in zebrafish and mice using a bright, photostable ratiometric fluorescent probe",

abstract = " A high brightness red fluorescent probe (S-BODIPY) has been developed for the sensitive and specific imaging of HClO/ClO - in vitro and in vivo. This probe exhibits some distinctive features such as excellent resistance to photobleaching, a high fluorescence brightness, high selectivity, as well as a good biocompatibility. Upon oxidation of the thio-ether group into sulfoxide, the probe showed a noticeable ratiometric fluorescence response toward ClO - with fast response (within 30 s) and a low detection limit (59 nM). The probe demonstrated the successful imaging of exogenous and endogenous HClO/ClO - in living HeLa cells, zebrafish, and mice with high signal-to-noise ratios. S-BODIPY allows for the real-time monitoring the level of ClO - in living cells by ratiometric fluorescence imaging, opening up exciting prospects to develop red and even near-infrared BODIPYs with high brightness and good photostability for in vivo imaging. ",

author = "Chong Duan and Miae Won and Peter Verwilst and Junchao Xu and Kim, {Hyeong Seok} and Lintao Zeng and Kim, {Jong Seung}",

note = "Funding Information: 2018R1A3B1052702, J.S.K.), and the Basic Science Research Program (2017R1D1A1B03032561, P.V. and 2017R1D1A1B03030062, M.W.) funded by the Ministry of Education as well as the Korea Research Fellowship Program funded by the Ministry of Science and ICT through the National Research Foundation of Korea (2016H1D3A1938052, P.V.). Funding Information: We gratefully thank the financial support from the National Natural Science Foundation of China (No.21203138, L.Z.) and the Natural Science Foundation of Tianjin (17JCYBJC19600, L.Z.). This work was also supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (CRI project no. Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",

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AU - Duan, Chong

AU - Won, Miae

AU - Verwilst, Peter

AU - Xu, Junchao

AU - Kim, Hyeong Seok

AU - Zeng, Lintao

AU - Kim, Jong Seung

N1 - Funding Information: 2018R1A3B1052702, J.S.K.), and the Basic Science Research Program (2017R1D1A1B03032561, P.V. and 2017R1D1A1B03030062, M.W.) funded by the Ministry of Education as well as the Korea Research Fellowship Program funded by the Ministry of Science and ICT through the National Research Foundation of Korea (2016H1D3A1938052, P.V.). Funding Information: We gratefully thank the financial support from the National Natural Science Foundation of China (No.21203138, L.Z.) and the Natural Science Foundation of Tianjin (17JCYBJC19600, L.Z.). This work was also supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (CRI project no. Publisher Copyright: © 2019 American Chemical Society.

PY - 2019/3/19

Y1 - 2019/3/19

N2 - A high brightness red fluorescent probe (S-BODIPY) has been developed for the sensitive and specific imaging of HClO/ClO - in vitro and in vivo. This probe exhibits some distinctive features such as excellent resistance to photobleaching, a high fluorescence brightness, high selectivity, as well as a good biocompatibility. Upon oxidation of the thio-ether group into sulfoxide, the probe showed a noticeable ratiometric fluorescence response toward ClO - with fast response (within 30 s) and a low detection limit (59 nM). The probe demonstrated the successful imaging of exogenous and endogenous HClO/ClO - in living HeLa cells, zebrafish, and mice with high signal-to-noise ratios. S-BODIPY allows for the real-time monitoring the level of ClO - in living cells by ratiometric fluorescence imaging, opening up exciting prospects to develop red and even near-infrared BODIPYs with high brightness and good photostability for in vivo imaging.

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In vivo imaging of endogenously produced HCLO in zebrafish and mice using a bright, photostable ratiometric fluorescent probe

Abstract

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