In vivo imaging of endogenously produced HCLO in zebrafish and mice using a bright, photostable ratiometric fluorescent probe

Chong Duan, Miae Won, Peter Verwilst, Junchao Xu, Hyeong Seok Kim, Lintao Zeng, Jong Seung Kim

Research output: Contribution to journalArticlepeer-review

247 Citations (Scopus)

Abstract

A high brightness red fluorescent probe (S-BODIPY) has been developed for the sensitive and specific imaging of HClO/ClO - in vitro and in vivo. This probe exhibits some distinctive features such as excellent resistance to photobleaching, a high fluorescence brightness, high selectivity, as well as a good biocompatibility. Upon oxidation of the thio-ether group into sulfoxide, the probe showed a noticeable ratiometric fluorescence response toward ClO - with fast response (within 30 s) and a low detection limit (59 nM). The probe demonstrated the successful imaging of exogenous and endogenous HClO/ClO - in living HeLa cells, zebrafish, and mice with high signal-to-noise ratios. S-BODIPY allows for the real-time monitoring the level of ClO - in living cells by ratiometric fluorescence imaging, opening up exciting prospects to develop red and even near-infrared BODIPYs with high brightness and good photostability for in vivo imaging.

Original languageEnglish
Pages (from-to)4172-4178
Number of pages7
JournalAnalytical chemistry
Volume91
Issue number6
DOIs
Publication statusPublished - 2019 Mar 19

Bibliographical note

Funding Information:
2018R1A3B1052702, J.S.K.), and the Basic Science Research Program (2017R1D1A1B03032561, P.V. and 2017R1D1A1B03030062, M.W.) funded by the Ministry of Education as well as the Korea Research Fellowship Program funded by the Ministry of Science and ICT through the National Research Foundation of Korea (2016H1D3A1938052, P.V.).

Funding Information:
We gratefully thank the financial support from the National Natural Science Foundation of China (No.21203138, L.Z.) and the Natural Science Foundation of Tianjin (17JCYBJC19600, L.Z.). This work was also supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (CRI project no.

Publisher Copyright:
© 2019 American Chemical Society.

ASJC Scopus subject areas

  • Analytical Chemistry

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