In vivo imaging of near-membrane calcium ions with two-photon probes

Chang Su Lim, Min Young Kang, Ji Hee Han, Isravel Antony Danish, Bong Rae Cho

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


We report a two-photon (TP) probe (ACaLN) for near-membrane Ca2+ that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca2+, dissociation constants (KdTP) of (1.9±0.2) μM, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca2+ in live cells for more than 1500 s and in living tissues at 120 μm depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.

Original languageEnglish
Pages (from-to)2028-2033
Number of pages6
JournalChemistry - An Asian Journal
Issue number8
Publication statusPublished - 2011 Aug 1


  • calcium
  • fluorescence
  • microscopy
  • nonlinear spectroscopy
  • two-photon probe

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry


Dive into the research topics of 'In vivo imaging of near-membrane calcium ions with two-photon probes'. Together they form a unique fingerprint.

Cite this