In vivo imaging of near-membrane calcium ions with two-photon probes

Chang Su Lim; Min Young Kang; Ji Hee Han; Isravel Antony Danish; Bong Rae Cho

doi:10.1002/asia.201100156

In vivo imaging of near-membrane calcium ions with two-photon probes

Chang Su Lim
, Min Young Kang
, Ji Hee Han
, Isravel Antony Danish
, Bong Rae Cho^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

We report a two-photon (TP) probe (ACaLN) for near-membrane Ca²⁺ that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca²⁺, dissociation constants (K_d^TP) of (1.9±0.2) μM, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca²⁺ in live cells for more than 1500 s and in living tissues at 120 μm depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.

Original language	English
Pages (from-to)	2028-2033
Number of pages	6
Journal	Chemistry - An Asian Journal
Volume	6
Issue number	8
DOIs	https://doi.org/10.1002/asia.201100156
Publication status	Published - 2011 Aug 1

Keywords

calcium
fluorescence
microscopy
nonlinear spectroscopy
two-photon probe

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

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10.1002/asia.201100156

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title = "In vivo imaging of near-membrane calcium ions with two-photon probes",

abstract = "We report a two-photon (TP) probe (ACaLN) for near-membrane Ca2+ that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca2+, dissociation constants (KdTP) of (1.9±0.2) μM, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca2+ in live cells for more than 1500 s and in living tissues at 120 μm depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.",

keywords = "calcium, fluorescence, microscopy, nonlinear spectroscopy, two-photon probe",

author = "Lim, \{Chang Su\} and Kang, \{Min Young\} and Han, \{Ji Hee\} and Danish, \{Isravel Antony\} and Cho, \{Bong Rae\}",

year = "2011",

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doi = "10.1002/asia.201100156",

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T1 - In vivo imaging of near-membrane calcium ions with two-photon probes

AU - Lim, Chang Su

AU - Kang, Min Young

AU - Han, Ji Hee

AU - Danish, Isravel Antony

AU - Cho, Bong Rae

PY - 2011/8/1

Y1 - 2011/8/1

N2 - We report a two-photon (TP) probe (ACaLN) for near-membrane Ca2+ that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca2+, dissociation constants (KdTP) of (1.9±0.2) μM, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca2+ in live cells for more than 1500 s and in living tissues at 120 μm depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.

AB - We report a two-photon (TP) probe (ACaLN) for near-membrane Ca2+ that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca2+, dissociation constants (KdTP) of (1.9±0.2) μM, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca2+ in live cells for more than 1500 s and in living tissues at 120 μm depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.

KW - calcium

KW - fluorescence

KW - microscopy

KW - nonlinear spectroscopy

KW - two-photon probe

UR - https://www.scopus.com/pages/publications/79961043510

UR - https://www.scopus.com/pages/publications/79961043510#tab=citedBy

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M3 - Article

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AN - SCOPUS:79961043510

SN - 1861-4728

VL - 6

SP - 2028

EP - 2033

JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

IS - 8

ER -

In vivo imaging of near-membrane calcium ions with two-photon probes

Abstract

Keywords

ASJC Scopus subject areas

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