Inactivation of Salmonella enterica in chicken feces on the surface of eggshells by simultaneous treatments with gaseous chlorine dioxide and mild wet heat

Sunhyung Park; Larry R. Beuchat; Hoikyung Kim; Jee Hoon Ryu

doi:10.1016/j.fm.2016.10.026

Inactivation of Salmonella enterica in chicken feces on the surface of eggshells by simultaneous treatments with gaseous chlorine dioxide and mild wet heat

Sunhyung Park, Larry R. Beuchat, Hoikyung Kim, Jee Hoon Ryu

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The aim of this study was to investigate the lethal effects of simultaneous treatments with gaseous chlorine dioxide (ClO₂) and mild wet heat (55 °C at 100% relative humidity [RH]) on Salmonella enterica in chicken feces on the surface of eggshells. Gaseous ClO₂ production decreased significantly (P ≤ 0.05) as the RH (23, 43, 68, 85, and 100%) at 25 °C was increased. The lethality of gaseous ClO₂ against S. enterica in feces on eggshells increased significantly (P ≤ 0.05) as RH increased. For example, when treated with gaseous ClO₂ at 85 and 100% RH at 25 °C, S. enterica (5.9 log CFU/egg) was inactivated within 4 h. In contrast, at 23, 43, and 68% RH, the pathogen remained at 5.1, 5.0, and 2.8 log CFU/egg, respectively, after 6 h. Finally, when eggshells surface-contaminated with S. enterica (5.8 log CFU/egg) were treated with gaseous ClO₂ (peak concentration of ClO₂: 185.6 ppm) at 100% RH and 55 °C, inactivation occurred within 1 h. These results indicate that treatment of surface-contaminated shell eggs with gaseous ClO₂ at elevated RH and temperature is effective in inactivating S. enterica. These observations will be useful when developing an effective sanitation program to enhance the microbiological safety of shell eggs.

Original language	English
Pages (from-to)	202-206
Number of pages	5
Journal	Food Microbiology
Volume	62
DOIs	https://doi.org/10.1016/j.fm.2016.10.026
Publication status	Published - 2017 Apr 1

Bibliographical note

Funding Information:
This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) ( NRF- 2012R1A1A2007954 ).

Publisher Copyright:
© 2016 Elsevier Ltd

Keywords

Eggshell
Gaseous chlorine dioxide
High relative humidity
High temperature
Salmonella enterica
Sanitizer

ASJC Scopus subject areas

Food Science
Microbiology

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10.1016/j.fm.2016.10.026

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@article{b7094670e74744c199b7c8bf02bd121d,

title = "Inactivation of Salmonella enterica in chicken feces on the surface of eggshells by simultaneous treatments with gaseous chlorine dioxide and mild wet heat",

abstract = "The aim of this study was to investigate the lethal effects of simultaneous treatments with gaseous chlorine dioxide (ClO2) and mild wet heat (55 °C at 100% relative humidity [RH]) on Salmonella enterica in chicken feces on the surface of eggshells. Gaseous ClO2 production decreased significantly (P ≤ 0.05) as the RH (23, 43, 68, 85, and 100%) at 25 °C was increased. The lethality of gaseous ClO2 against S. enterica in feces on eggshells increased significantly (P ≤ 0.05) as RH increased. For example, when treated with gaseous ClO2 at 85 and 100% RH at 25 °C, S. enterica (5.9 log CFU/egg) was inactivated within 4 h. In contrast, at 23, 43, and 68% RH, the pathogen remained at 5.1, 5.0, and 2.8 log CFU/egg, respectively, after 6 h. Finally, when eggshells surface-contaminated with S. enterica (5.8 log CFU/egg) were treated with gaseous ClO2 (peak concentration of ClO2: 185.6 ppm) at 100% RH and 55 °C, inactivation occurred within 1 h. These results indicate that treatment of surface-contaminated shell eggs with gaseous ClO2 at elevated RH and temperature is effective in inactivating S. enterica. These observations will be useful when developing an effective sanitation program to enhance the microbiological safety of shell eggs.",

keywords = "Eggshell, Gaseous chlorine dioxide, High relative humidity, High temperature, Salmonella enterica, Sanitizer",

author = "Sunhyung Park and Beuchat, {Larry R.} and Hoikyung Kim and Ryu, {Jee Hoon}",

note = "Funding Information: This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) ( NRF- 2012R1A1A2007954 ). Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd",

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doi = "10.1016/j.fm.2016.10.026",

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AU - Park, Sunhyung

AU - Beuchat, Larry R.

AU - Kim, Hoikyung

AU - Ryu, Jee Hoon

N1 - Funding Information: This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) ( NRF- 2012R1A1A2007954 ). Publisher Copyright: © 2016 Elsevier Ltd

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N2 - The aim of this study was to investigate the lethal effects of simultaneous treatments with gaseous chlorine dioxide (ClO2) and mild wet heat (55 °C at 100% relative humidity [RH]) on Salmonella enterica in chicken feces on the surface of eggshells. Gaseous ClO2 production decreased significantly (P ≤ 0.05) as the RH (23, 43, 68, 85, and 100%) at 25 °C was increased. The lethality of gaseous ClO2 against S. enterica in feces on eggshells increased significantly (P ≤ 0.05) as RH increased. For example, when treated with gaseous ClO2 at 85 and 100% RH at 25 °C, S. enterica (5.9 log CFU/egg) was inactivated within 4 h. In contrast, at 23, 43, and 68% RH, the pathogen remained at 5.1, 5.0, and 2.8 log CFU/egg, respectively, after 6 h. Finally, when eggshells surface-contaminated with S. enterica (5.8 log CFU/egg) were treated with gaseous ClO2 (peak concentration of ClO2: 185.6 ppm) at 100% RH and 55 °C, inactivation occurred within 1 h. These results indicate that treatment of surface-contaminated shell eggs with gaseous ClO2 at elevated RH and temperature is effective in inactivating S. enterica. These observations will be useful when developing an effective sanitation program to enhance the microbiological safety of shell eggs.

AB - The aim of this study was to investigate the lethal effects of simultaneous treatments with gaseous chlorine dioxide (ClO2) and mild wet heat (55 °C at 100% relative humidity [RH]) on Salmonella enterica in chicken feces on the surface of eggshells. Gaseous ClO2 production decreased significantly (P ≤ 0.05) as the RH (23, 43, 68, 85, and 100%) at 25 °C was increased. The lethality of gaseous ClO2 against S. enterica in feces on eggshells increased significantly (P ≤ 0.05) as RH increased. For example, when treated with gaseous ClO2 at 85 and 100% RH at 25 °C, S. enterica (5.9 log CFU/egg) was inactivated within 4 h. In contrast, at 23, 43, and 68% RH, the pathogen remained at 5.1, 5.0, and 2.8 log CFU/egg, respectively, after 6 h. Finally, when eggshells surface-contaminated with S. enterica (5.8 log CFU/egg) were treated with gaseous ClO2 (peak concentration of ClO2: 185.6 ppm) at 100% RH and 55 °C, inactivation occurred within 1 h. These results indicate that treatment of surface-contaminated shell eggs with gaseous ClO2 at elevated RH and temperature is effective in inactivating S. enterica. These observations will be useful when developing an effective sanitation program to enhance the microbiological safety of shell eggs.

KW - Eggshell

KW - Gaseous chlorine dioxide

KW - High relative humidity

KW - High temperature

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KW - Sanitizer

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ER -

Inactivation of Salmonella enterica in chicken feces on the surface of eggshells by simultaneous treatments with gaseous chlorine dioxide and mild wet heat

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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