Increase of multidrug efflux pump expression in fluoroquinoloneresistant Salmonella mutants induced by ciprofloxacin selective pressure

Hyun Wol Kang, Gun Jo Woo

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Multidrug-resistant foodborne pathogens are a leading public health concern, as antimicrobial resistance can lead to therapeutic failure. In this study, a ciprofloxacin-susceptible Salmonella Istanbul (Sal10- FC-KU12) was isolated from chicken meat obtained from a market in Korea to induce ciprofloxacinresistant mutants (SalML, SalMM, and SalMH). Minimum inhibitory concentrations (MICs) of 12 antibiotics were measured in the presence or absence of an efflux pump inhibitor. Expression levels of efflux pumprelated genes (acrB, acrF, marA, ramA, rob, and soxS)were determined using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Elevated MICs for the derived mutants were shown to result from the action of the efflux pump, with increased expression of marA, ramA, and acrB compared with the wild-type strain. The results of this study suggest that continued use of ciprofloxacin might induce the emergence of Salmonella mutants resistant not only to fluoroquinolones, but also to several other classes of antimicrobials.

Original languageEnglish
Pages (from-to)182-186
Number of pages5
JournalResearch in Veterinary Science
Volume97
Issue number2
DOIs
Publication statusPublished - 2014

Bibliographical note

Funding Information:
This work was supported by grants from the Brain Korea 21 (BK21, T1300291), National Antimicrobial Resistance Management Program ( 11092NARMP158 ) of the Ministry of Food and Drug Safety , and Rural Development Administration (RDA, PJ007585 ). We would also like to thank the Korea University Food Safety Hall and Institute of Food and Biomedicine Safety for allowing the use of their equipment and facilities.

Keywords

  • Antimicrobial resistance
  • Efflux pump
  • Fluoroquinolone
  • Salmonella enterica

ASJC Scopus subject areas

  • General Veterinary

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