Abstract
The natural microorganism Escherichia coli without modification is not suitable for the efficient production of 2,3-butanediol (2,3-BD) on an industrial scale because of its poor metabolic performance. Metabolic capacities of E. coli have been improved to produce 2,3-BD efficiently, the performance of which is possible for producing such a product. Codon optimization with the ribosome-binding site for the efficient production of target genes (budA and budC) was achieved by molecular engineering, which allowed the metabolic engineering to proceed to the next level. As a result, comparing the productivity in 26 H, where the amount of p18COR was 1.04 g/L and that of p18WTR was 0.41 g/L, represents an approximate 60.6% increase in the productivity of the p18WTR with codon optimization. In other words, p18COR was 2.54-fold greater than p18WTR in the production of 2,3-BD.
Original language | English |
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Pages (from-to) | 535-540 |
Number of pages | 6 |
Journal | Biotechnology and Applied Biochemistry |
Volume | 61 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2014 Sept 1 |
Keywords
- 2 3-butanediol
- Escherichia coli
- acetoin reductase
- acetolactate decarboxylase
- codon optimization
- ribosome-binding site
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Drug Discovery
- Bioengineering
- Molecular Medicine
- Process Chemistry and Technology
- Biotechnology
- Biomedical Engineering