Increased HOXC6 mRNA expression is a novel biomarker of gastric cancer

Jiyoon Jung; Sanghoon Jeong; Hoiseon Jeong; Hwa Eun Oh; Jung Woo Choi; Eung Seok Lee; Young Sik Kim; Yoonjin Kwak; Woo Ho Kim; Ju Han Lee

doi:10.1371/journal.pone.0236811

Increased HOXC6 mRNA expression is a novel biomarker of gastric cancer

Jiyoon Jung, Sanghoon Jeong, Hoiseon Jeong, Hwa Eun Oh, Jung Woo Choi, Eung Seok Lee, Young Sik Kim, Yoonjin Kwak, Woo Ho Kim, Ju Han Lee

Department of Medicine

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

In this study, we aimed to investigate the molecular biomarkers that are pivotal for the development and progression of gastric cancer (GC). We analyzed clinical specimens using RNA sequencing to identify the target genes. We found that the expression of HOXC6 mRNA was upregulated with the progression of cancer, which was validated by quantitative real time PCR and RNA in-situ hybridization. To compare the protein expression of HOXC6, we evaluated GC and normal gastric tissue samples using western blot analysis and immunohistochemistry. We detected significantly higher levels of HOXC6 in the GC tissues than in the normal controls at both mRNA and protein levels. The expression levels of HOXC6 mRNA in patients with advanced gastric cancer (AGC) were significantly higher than those in patients with early gastric cancer (EGC). Kaplan-Meier curves showed that high expression of HOXC6 mRNA is significantly associated with poor clinical prognosis. Our findings suggest that HOXC6 mRNA may be a novel biomarker and can be potentially valuable in predicting the prognosis of GC patients. Especially, HOXC6 mRNA in-situ hybridization may be a diagnostic tool for predicting prognosis of individual GC patients.

Original language	English
Article number	e0236811
Journal	PloS one
Volume	15
Issue number	8 August
DOIs	https://doi.org/10.1371/journal.pone.0236811
Publication status	Published - 2020 Aug

Bibliographical note

Publisher Copyright:
© 2020 Jung et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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title = "Increased HOXC6 mRNA expression is a novel biomarker of gastric cancer",

abstract = "In this study, we aimed to investigate the molecular biomarkers that are pivotal for the development and progression of gastric cancer (GC). We analyzed clinical specimens using RNA sequencing to identify the target genes. We found that the expression of HOXC6 mRNA was upregulated with the progression of cancer, which was validated by quantitative real time PCR and RNA in-situ hybridization. To compare the protein expression of HOXC6, we evaluated GC and normal gastric tissue samples using western blot analysis and immunohistochemistry. We detected significantly higher levels of HOXC6 in the GC tissues than in the normal controls at both mRNA and protein levels. The expression levels of HOXC6 mRNA in patients with advanced gastric cancer (AGC) were significantly higher than those in patients with early gastric cancer (EGC). Kaplan-Meier curves showed that high expression of HOXC6 mRNA is significantly associated with poor clinical prognosis. Our findings suggest that HOXC6 mRNA may be a novel biomarker and can be potentially valuable in predicting the prognosis of GC patients. Especially, HOXC6 mRNA in-situ hybridization may be a diagnostic tool for predicting prognosis of individual GC patients.",

author = "Jiyoon Jung and Sanghoon Jeong and Hoiseon Jeong and Oh, {Hwa Eun} and Choi, {Jung Woo} and Lee, {Eung Seok} and Kim, {Young Sik} and Yoonjin Kwak and Kim, {Woo Ho} and Lee, {Ju Han}",

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AU - Jung, Jiyoon

AU - Jeong, Sanghoon

AU - Jeong, Hoiseon

AU - Oh, Hwa Eun

AU - Choi, Jung Woo

AU - Lee, Eung Seok

AU - Kim, Young Sik

AU - Kwak, Yoonjin

AU - Kim, Woo Ho

AU - Lee, Ju Han

N1 - Publisher Copyright: © 2020 Jung et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2020/8

Y1 - 2020/8

N2 - In this study, we aimed to investigate the molecular biomarkers that are pivotal for the development and progression of gastric cancer (GC). We analyzed clinical specimens using RNA sequencing to identify the target genes. We found that the expression of HOXC6 mRNA was upregulated with the progression of cancer, which was validated by quantitative real time PCR and RNA in-situ hybridization. To compare the protein expression of HOXC6, we evaluated GC and normal gastric tissue samples using western blot analysis and immunohistochemistry. We detected significantly higher levels of HOXC6 in the GC tissues than in the normal controls at both mRNA and protein levels. The expression levels of HOXC6 mRNA in patients with advanced gastric cancer (AGC) were significantly higher than those in patients with early gastric cancer (EGC). Kaplan-Meier curves showed that high expression of HOXC6 mRNA is significantly associated with poor clinical prognosis. Our findings suggest that HOXC6 mRNA may be a novel biomarker and can be potentially valuable in predicting the prognosis of GC patients. Especially, HOXC6 mRNA in-situ hybridization may be a diagnostic tool for predicting prognosis of individual GC patients.

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Increased HOXC6 mRNA expression is a novel biomarker of gastric cancer

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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