Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli

Sang Jun Sim; Kristi D. Snell; Byung Woo Kim; Cho Kyun Rha; Anthony J. Sinskey

doi:10.1023/A:1013752000022

Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli

Sang Jun Sim, Kristi D. Snell, Byung Woo Kim, Cho Kyun Rha, Anthony J. Sinskey

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A second promoter (PI) was inserted to the PHA (poly-β-hydroxyalkanoate) operon (pSP2) of Esherichia coli DH5α with an optimal E. coli ribosome binding site and a trc strong promoter (pSJS1) to obtain poly-β-hydroxybutyrate (PHB) with long chain length. When the inducer, IPTG was added to the culture at 0.4 mM, the average molecular weight was 1.1 × 10⁶ Da. However, an even greater increase of the PHB average molecular weight to 2.5 × 10⁷ Da was observed without IPTG being added.

Original language	English
Pages (from-to)	2057-2061
Number of pages	5
Journal	Biotechnology letters
Volume	23
Issue number	24
DOIs	https://doi.org/10.1023/A:1013752000022
Publication status	Published - 2001

Keywords

Average molecular weight
PHA synthase
Polyhydroxybutyrate

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1023/A:1013752000022

Cite this

@article{c90493a3432a48289e9c9099294594f9,

title = "Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli",

abstract = "A second promoter (PI) was inserted to the PHA (poly-β-hydroxyalkanoate) operon (pSP2) of Esherichia coli DH5α with an optimal E. coli ribosome binding site and a trc strong promoter (pSJS1) to obtain poly-β-hydroxybutyrate (PHB) with long chain length. When the inducer, IPTG was added to the culture at 0.4 mM, the average molecular weight was 1.1 × 106 Da. However, an even greater increase of the PHB average molecular weight to 2.5 × 107 Da was observed without IPTG being added.",

keywords = "Average molecular weight, PHA synthase, Polyhydroxybutyrate",

author = "Sim, {Sang Jun} and Snell, {Kristi D.} and Kim, {Byung Woo} and Rha, {Cho Kyun} and Sinskey, {Anthony J.}",

year = "2001",

doi = "10.1023/A:1013752000022",

language = "English",

volume = "23",

pages = "2057--2061",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "24",

}

TY - JOUR

T1 - Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli

AU - Sim, Sang Jun

AU - Snell, Kristi D.

AU - Kim, Byung Woo

AU - Rha, Cho Kyun

AU - Sinskey, Anthony J.

PY - 2001

Y1 - 2001

N2 - A second promoter (PI) was inserted to the PHA (poly-β-hydroxyalkanoate) operon (pSP2) of Esherichia coli DH5α with an optimal E. coli ribosome binding site and a trc strong promoter (pSJS1) to obtain poly-β-hydroxybutyrate (PHB) with long chain length. When the inducer, IPTG was added to the culture at 0.4 mM, the average molecular weight was 1.1 × 106 Da. However, an even greater increase of the PHB average molecular weight to 2.5 × 107 Da was observed without IPTG being added.

AB - A second promoter (PI) was inserted to the PHA (poly-β-hydroxyalkanoate) operon (pSP2) of Esherichia coli DH5α with an optimal E. coli ribosome binding site and a trc strong promoter (pSJS1) to obtain poly-β-hydroxybutyrate (PHB) with long chain length. When the inducer, IPTG was added to the culture at 0.4 mM, the average molecular weight was 1.1 × 106 Da. However, an even greater increase of the PHB average molecular weight to 2.5 × 107 Da was observed without IPTG being added.

KW - Average molecular weight

KW - PHA synthase

KW - Polyhydroxybutyrate

UR - http://www.scopus.com/inward/record.url?scp=0035693242&partnerID=8YFLogxK

U2 - 10.1023/A:1013752000022

DO - 10.1023/A:1013752000022

M3 - Article

AN - SCOPUS:0035693242

SN - 0141-5492

VL - 23

SP - 2057

EP - 2061

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 24

ER -

Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this