Induction of human promyelocytic leukemia HL-60 cell differentiation into monocytes by silibinin: Involvement of protein kinase C

So N. Kang; Mi H. Lee; Kyeong Man Kim; Daeho Cho; Tae S. Kim

doi:10.1016/S0006-2952(01)00626-8

Induction of human promyelocytic leukemia HL-60 cell differentiation into monocytes by silibinin: Involvement of protein kinase C

So N. Kang
, Mi H. Lee
, Kyeong Man Kim
, Daeho Cho
, Tae S. Kim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

The effect of silibinin, an active component of Silybum marianum, on cellular differentiation was investigated in the human promyelocytic leukemia HL-60 cell culture system. Treatment of HL-60 cells with silibinin inhibited cellular proliferation and induced cellular differentiation in a dose-dependent manner. Cytofluorometric analysis and morphologic studies indicated that silibinin induced differentiation of HL-60 cells predominantly into monocytes. Importantly, strongly synergistic induction of differentiation into monocytes was observed when silibinin was combined with 5 nM 1α,25-dihydroxyvitamin D₃ [1,25-(OH)₂D₃], a well-known differentiation inducer of HL-60 cells into the monocytic lineage. Silibinin enhanced protein kinase C (PKC) activity and increased protein levels of both PKCα and PKCβ in 1,25-(OH)₂D₃-treated HL-60 cells. PKC and extracellular signal-regulated kinase (ERK) inhibitors significantly inhibited HL-60 cell differentiation induced by silibinin alone or in combination with 1,25-(OH)₂D₃, indicating that PKC and ERK may be involved in silibinin-induced HL-60 cell differentiation.

Original language	English
Pages (from-to)	1487-1495
Number of pages	9
Journal	Biochemical Pharmacology
Volume	61
Issue number	12
DOIs	https://doi.org/10.1016/S0006-2952(01)00626-8
Publication status	Published - 2001 Jun 15
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank Drs. J.W. Lee, H.J. Han, and J.H. Cheong (all with Chonnam National University, Korea) for providing valuable reagents and instruments. This work was supported by grants from the Korea Science and Engineering Foundation (HRC 1998G0021) and in part from the GenoCheck Co., Ltd (Ansan, Korea) (GC 20001).

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

1α,25-Dihydroxyvitamin D
Extracellular signal-regulated kinase
HL-60 cell differentiation
Protein kinase C
Silibinin

ASJC Scopus subject areas

Biochemistry
Pharmacology

Access to Document

10.1016/S0006-2952(01)00626-8

Cite this

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title = "Induction of human promyelocytic leukemia HL-60 cell differentiation into monocytes by silibinin: Involvement of protein kinase C",

abstract = "The effect of silibinin, an active component of Silybum marianum, on cellular differentiation was investigated in the human promyelocytic leukemia HL-60 cell culture system. Treatment of HL-60 cells with silibinin inhibited cellular proliferation and induced cellular differentiation in a dose-dependent manner. Cytofluorometric analysis and morphologic studies indicated that silibinin induced differentiation of HL-60 cells predominantly into monocytes. Importantly, strongly synergistic induction of differentiation into monocytes was observed when silibinin was combined with 5 nM 1α,25-dihydroxyvitamin D3 [1,25-(OH)2D3], a well-known differentiation inducer of HL-60 cells into the monocytic lineage. Silibinin enhanced protein kinase C (PKC) activity and increased protein levels of both PKCα and PKCβ in 1,25-(OH)2D3-treated HL-60 cells. PKC and extracellular signal-regulated kinase (ERK) inhibitors significantly inhibited HL-60 cell differentiation induced by silibinin alone or in combination with 1,25-(OH)2D3, indicating that PKC and ERK may be involved in silibinin-induced HL-60 cell differentiation.",

keywords = "1α,25-Dihydroxyvitamin D, Extracellular signal-regulated kinase, HL-60 cell differentiation, Protein kinase C, Silibinin",

author = "Kang, \{So N.\} and Lee, \{Mi H.\} and Kim, \{Kyeong Man\} and Daeho Cho and Kim, \{Tae S.\}",

note = "Funding Information: We would like to thank Drs. J.W. Lee, H.J. Han, and J.H. Cheong (all with Chonnam National University, Korea) for providing valuable reagents and instruments. This work was supported by grants from the Korea Science and Engineering Foundation (HRC 1998G0021) and in part from the GenoCheck Co., Ltd (Ansan, Korea) (GC 20001). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

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doi = "10.1016/S0006-2952(01)00626-8",

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AU - Cho, Daeho

AU - Kim, Tae S.

N1 - Funding Information: We would like to thank Drs. J.W. Lee, H.J. Han, and J.H. Cheong (all with Chonnam National University, Korea) for providing valuable reagents and instruments. This work was supported by grants from the Korea Science and Engineering Foundation (HRC 1998G0021) and in part from the GenoCheck Co., Ltd (Ansan, Korea) (GC 20001). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 2001/6/15

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N2 - The effect of silibinin, an active component of Silybum marianum, on cellular differentiation was investigated in the human promyelocytic leukemia HL-60 cell culture system. Treatment of HL-60 cells with silibinin inhibited cellular proliferation and induced cellular differentiation in a dose-dependent manner. Cytofluorometric analysis and morphologic studies indicated that silibinin induced differentiation of HL-60 cells predominantly into monocytes. Importantly, strongly synergistic induction of differentiation into monocytes was observed when silibinin was combined with 5 nM 1α,25-dihydroxyvitamin D3 [1,25-(OH)2D3], a well-known differentiation inducer of HL-60 cells into the monocytic lineage. Silibinin enhanced protein kinase C (PKC) activity and increased protein levels of both PKCα and PKCβ in 1,25-(OH)2D3-treated HL-60 cells. PKC and extracellular signal-regulated kinase (ERK) inhibitors significantly inhibited HL-60 cell differentiation induced by silibinin alone or in combination with 1,25-(OH)2D3, indicating that PKC and ERK may be involved in silibinin-induced HL-60 cell differentiation.

AB - The effect of silibinin, an active component of Silybum marianum, on cellular differentiation was investigated in the human promyelocytic leukemia HL-60 cell culture system. Treatment of HL-60 cells with silibinin inhibited cellular proliferation and induced cellular differentiation in a dose-dependent manner. Cytofluorometric analysis and morphologic studies indicated that silibinin induced differentiation of HL-60 cells predominantly into monocytes. Importantly, strongly synergistic induction of differentiation into monocytes was observed when silibinin was combined with 5 nM 1α,25-dihydroxyvitamin D3 [1,25-(OH)2D3], a well-known differentiation inducer of HL-60 cells into the monocytic lineage. Silibinin enhanced protein kinase C (PKC) activity and increased protein levels of both PKCα and PKCβ in 1,25-(OH)2D3-treated HL-60 cells. PKC and extracellular signal-regulated kinase (ERK) inhibitors significantly inhibited HL-60 cell differentiation induced by silibinin alone or in combination with 1,25-(OH)2D3, indicating that PKC and ERK may be involved in silibinin-induced HL-60 cell differentiation.

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KW - Extracellular signal-regulated kinase

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Induction of human promyelocytic leukemia HL-60 cell differentiation into monocytes by silibinin: Involvement of protein kinase C

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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