Induction of Id2 expression by cardiac transcription factors GATA4 and Nkx2.5

Joong Yeon Lim, Ho Kim Won, Joon Kim, Ick Park Sang

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    Inhibitor of differentiation/DNA binding (Id) proteins function as a regulator of helix-loop-helix proteins participating in cell lineage commitment and differentiation. Here, we observed a marked induction of Id2 during cardiomyocyte differentiation from P19CL6 murine embryonic teratocarcinoma stem cells, prompting us to investigate the upstream regulatory mechanism of Id2 induction. Computer analysis of Id2 promoter and subsequent electrophoretic mobility shift assay revealed several binding sites for GATA4 and Nkx2.5 within the Id2 promoter. By further deletion and mutation analysis of the respective binding site, we identified that two motifs located at -497/-502 and -264/-270 were functionally important for Id2 promoter activation by GATA4 and Nkx2.5, respectively. Overexpression of GATA4 and/or Nkx2.5 induced not only Id2 promoter activity but also Id2 protein expression. Additionally, Id proteins significantly inhibit the GATA4 and Nkx2.5-dependent transcription, suggesting Id proteins may play a regulatory role in cardiogenesis. Collectively, our results demonstrate that GATA4 and Nkx2.5 could be one of the upstream regulators of Id2.

    Original languageEnglish
    Pages (from-to)182-194
    Number of pages13
    JournalJournal of cellular biochemistry
    Volume103
    Issue number1
    DOIs
    Publication statusPublished - 2008 Jan 1

    Keywords

    • Cell differentiation
    • Gene expression regulation
    • Idb2 protein
    • Myocytes
    • Transcription factors

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Fingerprint

    Dive into the research topics of 'Induction of Id2 expression by cardiac transcription factors GATA4 and Nkx2.5'. Together they form a unique fingerprint.

    Cite this