Induction of in vivo persistent anti-mycobacterial activity by interferon-γ-secreting fibroblasts

Tae S. Kim; Su W. Chung; Bok Y. Kang; Yong Kyung Choe; Seung Y. Hwang

doi:10.1016/S0264-410X(99)00353-9

Induction of in vivo persistent anti-mycobacterial activity by interferon-γ-secreting fibroblasts

Tae S. Kim^*
, Su W. Chung
, Bok Y. Kang
, Yong Kyung Choe
, Seung Y. Hwang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

To determine whether the paracrine secretion of interferon-γ (IFN-γ) can efficiently stimulate the resistance to Mycobacterium avium complex (MAC) infection, 3T3 fibroblasts were stably transduced to secrete IFN-γ (500 units/10⁶ cells/48 h) and their effects on MAC infection were investigated in genetically susceptible BALB/c mice, compared with that of free recombinant IFN-γ (rIFN-γ). Immunization with IFN-γ-secreting fibroblasts (3T3-IFN-γ) during intranasal infection with MAC resulted in a significant decrease in bacterial load of lung during the entire 8-week observation period, while rIFN-γ reduced the bacterial load at initial 1 week but not by 8 weeks postinfection. Furthermore, immunization with the 3T3-IFN-γ cells induced and maintained significantly higher levels of cytotoxic activity and nitric oxide production by lung cells than those of rIFN-γ immunization. This work suggest that IFN-γ-secreting fibroblasts may serve as a vehicle for paracrine secretion of IFN-γ in immunotherapy of MAC infection.

Original language	English
Pages (from-to)	1067-1073
Number of pages	7
Journal	Vaccine
Volume	18
Issue number	11-12
DOIs	https://doi.org/10.1016/S0264-410X(99)00353-9
Publication status	Published - 2000 Jan 6
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank Dr. A, Bank, Dr. R. Mulligan, Dr. P. Gangadharam for providing valuable reagents. This work was supported by grants from the Korea Science and Engineering Foundation (IRC project to T.S.K.).

Keywords

IFN-γ-secreting fibroblast
Immunity
MAC

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0264-410X(99)00353-9

Cite this

@article{08542fd1d218485f9388a5f7d68f432a,

title = "Induction of in vivo persistent anti-mycobacterial activity by interferon-γ-secreting fibroblasts",

abstract = "To determine whether the paracrine secretion of interferon-γ (IFN-γ) can efficiently stimulate the resistance to Mycobacterium avium complex (MAC) infection, 3T3 fibroblasts were stably transduced to secrete IFN-γ (500 units/106 cells/48 h) and their effects on MAC infection were investigated in genetically susceptible BALB/c mice, compared with that of free recombinant IFN-γ (rIFN-γ). Immunization with IFN-γ-secreting fibroblasts (3T3-IFN-γ) during intranasal infection with MAC resulted in a significant decrease in bacterial load of lung during the entire 8-week observation period, while rIFN-γ reduced the bacterial load at initial 1 week but not by 8 weeks postinfection. Furthermore, immunization with the 3T3-IFN-γ cells induced and maintained significantly higher levels of cytotoxic activity and nitric oxide production by lung cells than those of rIFN-γ immunization. This work suggest that IFN-γ-secreting fibroblasts may serve as a vehicle for paracrine secretion of IFN-γ in immunotherapy of MAC infection.",

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author = "Kim, \{Tae S.\} and Chung, \{Su W.\} and Kang, \{Bok Y.\} and Choe, \{Yong Kyung\} and Hwang, \{Seung Y.\}",

note = "Funding Information: We would like to thank Dr. A, Bank, Dr. R. Mulligan, Dr. P. Gangadharam for providing valuable reagents. This work was supported by grants from the Korea Science and Engineering Foundation (IRC project to T.S.K.).",

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month = jan,

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doi = "10.1016/S0264-410X(99)00353-9",

language = "English",

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pages = "1067--1073",

journal = "Vaccine",

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TY - JOUR

T1 - Induction of in vivo persistent anti-mycobacterial activity by interferon-γ-secreting fibroblasts

AU - Kim, Tae S.

AU - Chung, Su W.

AU - Kang, Bok Y.

AU - Choe, Yong Kyung

AU - Hwang, Seung Y.

N1 - Funding Information: We would like to thank Dr. A, Bank, Dr. R. Mulligan, Dr. P. Gangadharam for providing valuable reagents. This work was supported by grants from the Korea Science and Engineering Foundation (IRC project to T.S.K.).

PY - 2000/1/6

Y1 - 2000/1/6

N2 - To determine whether the paracrine secretion of interferon-γ (IFN-γ) can efficiently stimulate the resistance to Mycobacterium avium complex (MAC) infection, 3T3 fibroblasts were stably transduced to secrete IFN-γ (500 units/106 cells/48 h) and their effects on MAC infection were investigated in genetically susceptible BALB/c mice, compared with that of free recombinant IFN-γ (rIFN-γ). Immunization with IFN-γ-secreting fibroblasts (3T3-IFN-γ) during intranasal infection with MAC resulted in a significant decrease in bacterial load of lung during the entire 8-week observation period, while rIFN-γ reduced the bacterial load at initial 1 week but not by 8 weeks postinfection. Furthermore, immunization with the 3T3-IFN-γ cells induced and maintained significantly higher levels of cytotoxic activity and nitric oxide production by lung cells than those of rIFN-γ immunization. This work suggest that IFN-γ-secreting fibroblasts may serve as a vehicle for paracrine secretion of IFN-γ in immunotherapy of MAC infection.

AB - To determine whether the paracrine secretion of interferon-γ (IFN-γ) can efficiently stimulate the resistance to Mycobacterium avium complex (MAC) infection, 3T3 fibroblasts were stably transduced to secrete IFN-γ (500 units/106 cells/48 h) and their effects on MAC infection were investigated in genetically susceptible BALB/c mice, compared with that of free recombinant IFN-γ (rIFN-γ). Immunization with IFN-γ-secreting fibroblasts (3T3-IFN-γ) during intranasal infection with MAC resulted in a significant decrease in bacterial load of lung during the entire 8-week observation period, while rIFN-γ reduced the bacterial load at initial 1 week but not by 8 weeks postinfection. Furthermore, immunization with the 3T3-IFN-γ cells induced and maintained significantly higher levels of cytotoxic activity and nitric oxide production by lung cells than those of rIFN-γ immunization. This work suggest that IFN-γ-secreting fibroblasts may serve as a vehicle for paracrine secretion of IFN-γ in immunotherapy of MAC infection.

KW - IFN-γ-secreting fibroblast

KW - Immunity

KW - MAC

UR - https://www.scopus.com/pages/publications/0033990812

U2 - 10.1016/S0264-410X(99)00353-9

DO - 10.1016/S0264-410X(99)00353-9

M3 - Article

C2 - 10590327

AN - SCOPUS:0033990812

SN - 0264-410X

VL - 18

SP - 1067

EP - 1073

JO - Vaccine

JF - Vaccine

IS - 11-12

ER -

Induction of in vivo persistent anti-mycobacterial activity by interferon-γ-secreting fibroblasts

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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