Induction of interleukin-8 production via nuclear factor-κB activation in human intestinal epithelial cells infected with Vibrio vulnificus

B. C. Lee, S. H. Kim, S. H. Choi, T. S. Kim

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


Vibrio vulnificus, a Gram-negative estuarine bacterium, is a causative agent of food-borne diseases, such as life-threatening septicaemia and wound infection disease. V. vulnificus penetrating into the epithelial barrier stimulates an inflammatory response in the adjacent mucosa. Therefore, interaction between V. vulnificus and epithelial cells is important for understanding of both the immunology of mucosal surfaces and V. vulnificus. In this study, we investigated the effect and action mechanism of V. vulnificus infection on production of interleukin (IL)-8, a proinflammatory cytokine, in human intestinal epithelial INT-407 cells. V. vulnificus infection significantly induced IL-8 production in a time- and multiplicity of infection (MOI)-dependent manner, as determined by human IL-8 enzyme-linked immunosorbent assay (ELISA). In addition, V. vulnificus infection significantly increased IL-8 mRNA levels in INT-407 cells, indicating that the increased IL-8 production by V. vulnificus occurred at the transcriptional level. V. vulnificus infection also enhanced IL-8 gene promoter activity in INT-407 cells transiently transfected with IL-8 promoter constructs, but this effect was impaired in INT-407 cells transfected with IL-8 promoter constructs deleted or mutated of a κB site. V. vulnificus infection increased the nuclear factor-kappaB (NF-κB) binding activity to a κB site and the degradation of IκB-α protein in a time- and a MOI-dependent manner. Furthermore, BAY11-7082, an inhibitor of NF-κB activation, significantly reduced the IL-8 production, NF-κB binding activity and IκB-α degradation induced by V. vulnificus infection. Taken together, these results indicate clearly that V. vulnificus infection significantly induces IL-8 production in human intestinal epithelial cells via NF-κB activation.

Original languageEnglish
Pages (from-to)506-515
Number of pages10
Issue number4
Publication statusPublished - 2005 Aug
Externally publishedYes


  • Epithelial cell
  • Interleukin-8
  • Nuclear factor-κB
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Induction of interleukin-8 production via nuclear factor-κB activation in human intestinal epithelial cells infected with Vibrio vulnificus'. Together they form a unique fingerprint.

Cite this