Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1

Jai Hee Moon; Byung Sun Yoon; Bona Kim; Gyuman Park; Hye Youn Jung; Isaac Maeng; Eun Kyoung Jun; Seung Jun Yoo; Aeree Kim; Sejong Oh; Kwang Youn Whang; Hyunggee Kim; Dong Wook Kim; Ki Dong Kim; Seungkwon You

doi:10.1016/j.bbrc.2008.04.068

Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1

Jai Hee Moon, Byung Sun Yoon, Bona Kim, Gyuman Park, Hye Youn Jung, Isaac Maeng, Eun Kyoung Jun, Seung Jun Yoo, Aeree Kim, Sejong Oh, Kwang Youn Whang, Hyunggee Kim, Dong Wook Kim, Ki Dong Kim, Seungkwon You

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1.

Original language	English
Pages (from-to)	267-272
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	371
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2008.04.068
Publication status	Published - 2008 Jun 27

Bibliographical note

Funding Information:
We thank Dr G.P. Dimri for his generous gift of the pBabe-Bmi1 and pBabe dominant-negative Bmi1 expression vector. This work was supported by a Research Grant from Imgen, Inc. This work was supported by Grants SC4001 from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Korea.

Keywords

Astrocytes
Dedifferentiation
Neural stem cell-like cells
Neural stem cells

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.04.068

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title = "Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1",

abstract = "Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1.",

keywords = "Astrocytes, Dedifferentiation, Neural stem cell-like cells, Neural stem cells",

author = "Moon, {Jai Hee} and Yoon, {Byung Sun} and Bona Kim and Gyuman Park and Jung, {Hye Youn} and Isaac Maeng and Jun, {Eun Kyoung} and Yoo, {Seung Jun} and Aeree Kim and Sejong Oh and Whang, {Kwang Youn} and Hyunggee Kim and Kim, {Dong Wook} and Kim, {Ki Dong} and Seungkwon You",

note = "Funding Information: We thank Dr G.P. Dimri for his generous gift of the pBabe-Bmi1 and pBabe dominant-negative Bmi1 expression vector. This work was supported by a Research Grant from Imgen, Inc. This work was supported by Grants SC4001 from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Korea. ",

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doi = "10.1016/j.bbrc.2008.04.068",

language = "English",

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AU - Moon, Jai Hee

AU - Yoon, Byung Sun

AU - Kim, Bona

AU - Park, Gyuman

AU - Jung, Hye Youn

AU - Maeng, Isaac

AU - Jun, Eun Kyoung

AU - Yoo, Seung Jun

AU - Kim, Aeree

AU - Oh, Sejong

AU - Whang, Kwang Youn

AU - Kim, Hyunggee

AU - Kim, Dong Wook

AU - Kim, Ki Dong

AU - You, Seungkwon

N1 - Funding Information: We thank Dr G.P. Dimri for his generous gift of the pBabe-Bmi1 and pBabe dominant-negative Bmi1 expression vector. This work was supported by a Research Grant from Imgen, Inc. This work was supported by Grants SC4001 from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Korea.

PY - 2008/6/27

Y1 - 2008/6/27

N2 - Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1.

AB - Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1.

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KW - Dedifferentiation

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Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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