Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1.
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 2008 Jun 27|
Bibliographical noteFunding Information:
We thank Dr G.P. Dimri for his generous gift of the pBabe-Bmi1 and pBabe dominant-negative Bmi1 expression vector. This work was supported by a Research Grant from Imgen, Inc. This work was supported by Grants SC4001 from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Korea.
- Neural stem cell-like cells
- Neural stem cells
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology