TY - JOUR
T1 - Induction of pepper cDNA encoding a lipid transfer protein during the resistance response to tobacco mosaic virus
AU - Park, Chang Jin
AU - Shin, Ryoung
AU - Park, Jeong Mee
AU - Lee, Gil Je
AU - You, Jin Sam
AU - Paek, Kyung Hee
N1 - Funding Information:
We thank members of the Paek laboratory for helpful discussion during the work and Dr Sangduk Kim for critical review of the manuscript. We also thank Dr Woo Taek Kim (Yonsei University, Korea) for valuable assistance in ethylene treatment. This study was supported partly by a G7 research grant from the Korea Ministry of Science and Technology, a grant from the Korea Ministry of Agriculture and Forestry, and grants from the Korea Science and Engineering Foundation to the Center for Plant Molecular Genetics and Breeding Research and the Research Center for the Development of Advanced Horticultural Technology.
PY - 2002
Y1 - 2002
N2 - Pepper (Capsicum annuum) plants exhibit hypersensitive response (HR) against infection by many tobamoviruses. A clone encoding a putative nonspecific lipid transfer protein (CaLTP1) was isolated by differential screening of a cDNA library from resistant pepper leaves when inoculated with tobacco mosaic virus (TMV) pathotype P0. The predicted amino acid sequence of CaLTP1 is highly similar to that of the other plant LTPs. Southern blot analysis showed that a small gene family of LTP-related sequences was present in the pepper genome. Transcripts homologous to CaLTP1 accumulated abundantly in old leaves and flowers. CaLTP1 expression was induced in the incompatible interaction with TMV-P0 but was not induced in the compatible interaction with TMV-P1,2. In correlation with the temporal progression of HR in the inoculated leaves, CaLTP1 transcripts started to accumulate at 24 h after TMV-P0 inoculation, reaching a maximal level at 48 h. A strain of Xanthomonas campestris pv. vesicatoria (Xcv) that carries the bacterial avirulence gene, avrBs2, was infiltrated into leaves of a pepper cultivar containing the Bs2 resistance gene. A marked induction of CaLTP1 expression was observed in Xcv-infiltrated leaves. Effects of exogenously applied abiotic elicitors on CaLTP1 expression were also examined. Salicylic acid caused a rapid accumulation of CaLTP1 transcripts in pepper leaves and ethephon treatment also induced the expression of the CaLTP1 gene. Transient expression in the detached pepper leaves by biolistic gene bombardment indicated that CaLTP1 is localized mostly at the plant cell surface, possibly in the cell wall. These results suggest possible role(s) for LTPs in plant defense against pathogens including viruses.
AB - Pepper (Capsicum annuum) plants exhibit hypersensitive response (HR) against infection by many tobamoviruses. A clone encoding a putative nonspecific lipid transfer protein (CaLTP1) was isolated by differential screening of a cDNA library from resistant pepper leaves when inoculated with tobacco mosaic virus (TMV) pathotype P0. The predicted amino acid sequence of CaLTP1 is highly similar to that of the other plant LTPs. Southern blot analysis showed that a small gene family of LTP-related sequences was present in the pepper genome. Transcripts homologous to CaLTP1 accumulated abundantly in old leaves and flowers. CaLTP1 expression was induced in the incompatible interaction with TMV-P0 but was not induced in the compatible interaction with TMV-P1,2. In correlation with the temporal progression of HR in the inoculated leaves, CaLTP1 transcripts started to accumulate at 24 h after TMV-P0 inoculation, reaching a maximal level at 48 h. A strain of Xanthomonas campestris pv. vesicatoria (Xcv) that carries the bacterial avirulence gene, avrBs2, was infiltrated into leaves of a pepper cultivar containing the Bs2 resistance gene. A marked induction of CaLTP1 expression was observed in Xcv-infiltrated leaves. Effects of exogenously applied abiotic elicitors on CaLTP1 expression were also examined. Salicylic acid caused a rapid accumulation of CaLTP1 transcripts in pepper leaves and ethephon treatment also induced the expression of the CaLTP1 gene. Transient expression in the detached pepper leaves by biolistic gene bombardment indicated that CaLTP1 is localized mostly at the plant cell surface, possibly in the cell wall. These results suggest possible role(s) for LTPs in plant defense against pathogens including viruses.
KW - Differential screening
KW - Hypersensitive response
KW - Lipid transfer protein
KW - Pepper (Capsicum annuum L.)
KW - Tobacco mosaic virus
KW - cDNA cloning
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U2 - 10.1023/A:1013383329361
DO - 10.1023/A:1013383329361
M3 - Article
C2 - 11855726
AN - SCOPUS:0036007719
SN - 0167-4412
VL - 48
SP - 243
EP - 254
JO - Plant Molecular Biology
JF - Plant Molecular Biology
IS - 3
ER -