Abstract
Transforming growth factor (TGF)-β-inducible gene-h3 (βig-h3) product is a secreted protein that is induced by TGF-β in several cell types and implicated in various tissue pathologies. The aims of this study were to determine the effect of TGF-β1 on βig-h3 expression in cultured astrocytes and to examine whether βig-h3 is expressed in the brain after traumatic injury. The results showed that βig-h3 mRNA and protein increased in response to TGF-β1 in U87 human astrocytoma cells and mouse cortical astrocytes. Treatment with other cytokines, including tumor necrosis factor-α and fibroblast growth factor-2, did not enhance the expression of βig-h3 in astrocytes. βig-h3 was significantly expressed in reactive astrocytes at the site of a stab wound in the cerebral cortex of adult rats. These results provide an insight into understanding a novel role for βig-h3 protein in the response of astrocytes to brain injury.
| Original language | English |
|---|---|
| Pages (from-to) | 57-64 |
| Number of pages | 8 |
| Journal | Molecular Brain Research |
| Volume | 107 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2002 Oct 30 |
| Externally published | Yes |
Bibliographical note
Funding Information:This study was supported by grant 2000-003-D00109 (to EHL) from the Korean Research Foundation, Seoul, Korea. We thank Jin-Yong Chung for assistance with the animal surgery.
Keywords
- Astrocyte
- Brain injury
- Gliosis
- TGF-β1
- Wound healing
- βig-h3
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience