Induction of total insensitivity to capsaicin and hypersensitivity to garlic extract in human by decreased expression of TRPV1

Joong Jean Park; Jaehee Lee; Myung Ah Kim; Seung Keun Back; Seung Kil Hong; Heung Sik Na

doi:10.1016/j.neulet.2006.10.046

Induction of total insensitivity to capsaicin and hypersensitivity to garlic extract in human by decreased expression of TRPV1

Joong Jean Park
, Jaehee Lee
, Myung Ah Kim
, Seung Keun Back
, Seung Kil Hong
, Heung Sik Na^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

TRPV1 is a cation channel which is activated by temperature (≥42 °C) and capsaicin. In the present study, we found a person with total insensitivity to capsaicin and attempted to unravel its causes. The expression levels of TRPV1 protein and mRNA in the cells of the person's buccal mucosa were less than half of those in a normal subject. Sequential analysis of mRNA and genomic DNA revealed several point mutations mostly in the second intron of the person's TRPV1. Interestingly, the subject showed hypersensitivity to garlic extract, but TRPA1 (allicin receptor) level was normal. These results suggest that the decreased expression of TRPV1 may be related to a functional knock out in capsaicin sensation and hypersensitivity to allicin in humans.

Original language	English
Pages (from-to)	87-91
Number of pages	5
Journal	Neuroscience Letters
Volume	411
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2006.10.046
Publication status	Published - 2007 Jan 10

Bibliographical note

Funding Information:
This work was supported by a grant R01-2004-000-10132-0 from the Basic Research Program of the Korea Science & Engineering Foundation, and by a grant (M103KV010015-06K2201-01510) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, the Republic of Korea. We thank Dr. Paul Garrity and Mark Rosenzweig for offering dTRPA1 antiserum.

Keywords

Allicin
Buccal mucosa
Capsaicin
TRPA1
TRPV1

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neulet.2006.10.046

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title = "Induction of total insensitivity to capsaicin and hypersensitivity to garlic extract in human by decreased expression of TRPV1",

abstract = "TRPV1 is a cation channel which is activated by temperature (≥42 °C) and capsaicin. In the present study, we found a person with total insensitivity to capsaicin and attempted to unravel its causes. The expression levels of TRPV1 protein and mRNA in the cells of the person's buccal mucosa were less than half of those in a normal subject. Sequential analysis of mRNA and genomic DNA revealed several point mutations mostly in the second intron of the person's TRPV1. Interestingly, the subject showed hypersensitivity to garlic extract, but TRPA1 (allicin receptor) level was normal. These results suggest that the decreased expression of TRPV1 may be related to a functional knock out in capsaicin sensation and hypersensitivity to allicin in humans.",

keywords = "Allicin, Buccal mucosa, Capsaicin, TRPA1, TRPV1",

author = "Park, \{Joong Jean\} and Jaehee Lee and Kim, \{Myung Ah\} and Back, \{Seung Keun\} and Hong, \{Seung Kil\} and Na, \{Heung Sik\}",

note = "Funding Information: This work was supported by a grant R01-2004-000-10132-0 from the Basic Research Program of the Korea Science \& Engineering Foundation, and by a grant (M103KV010015-06K2201-01510) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, the Republic of Korea. We thank Dr. Paul Garrity and Mark Rosenzweig for offering dTRPA1 antiserum.",

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doi = "10.1016/j.neulet.2006.10.046",

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T1 - Induction of total insensitivity to capsaicin and hypersensitivity to garlic extract in human by decreased expression of TRPV1

AU - Park, Joong Jean

AU - Lee, Jaehee

AU - Kim, Myung Ah

AU - Back, Seung Keun

AU - Hong, Seung Kil

AU - Na, Heung Sik

N1 - Funding Information: This work was supported by a grant R01-2004-000-10132-0 from the Basic Research Program of the Korea Science & Engineering Foundation, and by a grant (M103KV010015-06K2201-01510) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, the Republic of Korea. We thank Dr. Paul Garrity and Mark Rosenzweig for offering dTRPA1 antiserum.

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Y1 - 2007/1/10

N2 - TRPV1 is a cation channel which is activated by temperature (≥42 °C) and capsaicin. In the present study, we found a person with total insensitivity to capsaicin and attempted to unravel its causes. The expression levels of TRPV1 protein and mRNA in the cells of the person's buccal mucosa were less than half of those in a normal subject. Sequential analysis of mRNA and genomic DNA revealed several point mutations mostly in the second intron of the person's TRPV1. Interestingly, the subject showed hypersensitivity to garlic extract, but TRPA1 (allicin receptor) level was normal. These results suggest that the decreased expression of TRPV1 may be related to a functional knock out in capsaicin sensation and hypersensitivity to allicin in humans.

AB - TRPV1 is a cation channel which is activated by temperature (≥42 °C) and capsaicin. In the present study, we found a person with total insensitivity to capsaicin and attempted to unravel its causes. The expression levels of TRPV1 protein and mRNA in the cells of the person's buccal mucosa were less than half of those in a normal subject. Sequential analysis of mRNA and genomic DNA revealed several point mutations mostly in the second intron of the person's TRPV1. Interestingly, the subject showed hypersensitivity to garlic extract, but TRPA1 (allicin receptor) level was normal. These results suggest that the decreased expression of TRPV1 may be related to a functional knock out in capsaicin sensation and hypersensitivity to allicin in humans.

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KW - Capsaicin

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ER -

Induction of total insensitivity to capsaicin and hypersensitivity to garlic extract in human by decreased expression of TRPV1

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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