TY - GEN
T1 - Inertial microfluidic cell hydroporator (IMCH) for high-throughput & single-step intracellular molecule delivery
AU - Deng, Yanxiang
AU - Rada, Miran
AU - Kizer, Megan
AU - Wang, Xing
AU - Cheon, Dong Joo
AU - Chung, Aram J.
N1 - Publisher Copyright:
© 17CBMS-0001.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - We present a high-throughput and single-step inertial microfluidic cell hydroporator (iMCH) capable of delivering diverse molecules to various cell types with a throughput of >30,000 cells/sec. Our approach is comprised of three sequential processes: (1) Cell injection, where cell suspensions with macromolecules are injected into a microchannel, (2) Inertial cell positioning, where randomly incoming cells are aligned via fluid inertia, and (3) Molecule delivery, where cells deform with large strain upon collision at a T-junction, and the rapid mechanical deformation of cells creates transient membrane openings that facilitate diffusion of molecules into the cytosol.
AB - We present a high-throughput and single-step inertial microfluidic cell hydroporator (iMCH) capable of delivering diverse molecules to various cell types with a throughput of >30,000 cells/sec. Our approach is comprised of three sequential processes: (1) Cell injection, where cell suspensions with macromolecules are injected into a microchannel, (2) Inertial cell positioning, where randomly incoming cells are aligned via fluid inertia, and (3) Molecule delivery, where cells deform with large strain upon collision at a T-junction, and the rapid mechanical deformation of cells creates transient membrane openings that facilitate diffusion of molecules into the cytosol.
KW - Inertial microfluidic cell hydroporator (iMCH)
KW - Inertial microfluidics
KW - Intracellular delivery
UR - http://www.scopus.com/inward/record.url?scp=85079676522&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079676522&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85079676522
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 169
EP - 170
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -