Influences of quorum-quenching probiotic bacteria on the gut microbial community and immune function in weaning pigs

Jonggun Kim, Jaepil Kim, Younghoon Kim, Sangnam Oh, Minho Song, Jee Hwan Choe, Kwang Youn Whang, Kwang Hyun Kim, Sejong Oh

    Research output: Contribution to journalArticlepeer-review

    30 Citations (Scopus)

    Abstract

    The aim of this study is to investigate the dynamic gut microbial diversity in weaning swine after administering feed supplemented with probiotic bacteria that specifically inhibit the activity of quorum molecules. Initially, the universal quorum molecule autoinducer-2 (AI-2) bioassay results indicated that AI-2 activity was profoundly inhibited in enterohemorrhagic Escherichia coli (EHEC) O157:H7 in the presence of Lactobacillus acidophilus strain 30SC cell extract, although the growth of EHEC was not affected. Based on plate counting results, bacterial community analysis revealed a specific reduction in coliforms compared to the control, whereas the population of lactobacilli increased in weaning swine in in vivo trials. Supplementation with L. acidophilus strain 30SC did not affect the counts of other communities, such as total aerobes and yeast/mold. In addition, PCR-denaturing gradient gel electrophoresis analysis showed a significant difference in the 16S rRNA gene products after administering L. acidophilus strain 30SC. Selected bands were sequenced, and most of them were identified as uncultured bacterium clones or a Lactobacillus- and Bifidobacterium-specific community. Therefore, our results indicate that quorum-quenching probiotic bacteria can significantly modulate the gut microbiota of swine and these beneficial effects can contribute to the improvement of performance and health in the gastrointestinal tract of weaning pigs.

    Original languageEnglish
    Pages (from-to)412-422
    Number of pages11
    JournalAnimal Science Journal
    Volume89
    Issue number2
    DOIs
    Publication statusPublished - 2018 Feb 1

    Bibliographical note

    Funding Information:
    This work was supported by the Cooperative Research Program for Agriculture Science & Technology Development, Rural Development Administration, Republic of Korea (Project No. PJ01088202).

    Publisher Copyright:
    © 2017 Japanese Society of Animal Science

    Keywords

    • PCR-denaturing gradient gel electrophoresis (PCR-DGGE)
    • autoinducer-2
    • bacterial community
    • probiotic bacteria
    • quorum sensing

    ASJC Scopus subject areas

    • General Medicine

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