Inhibition of histone deacetylation alters Arabidopsis root growth in response to auxin via PIN1 degradation

Hoai Nguyen Nguyen; Jun Hyeok Kim; Chan Young Jeong; Suk Whan Hong; Hojoung Lee

doi:10.1007/s00299-013-1474-6

Inhibition of histone deacetylation alters Arabidopsis root growth in response to auxin via PIN1 degradation

Hoai Nguyen Nguyen, Jun Hyeok Kim, Chan Young Jeong, Suk Whan Hong, Hojoung Lee

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Key message: Our results showed the histone deacetylase inhibitors (HDIs) control root development in Arabidopsis via regulation of PIN1 degradation. Epigenetic regulation plays a crucial role in the expression of many genes in response to exogenous or endogenous signals in plants as well as other organisms. One of epigenetic mechanisms is modifications of histone, such as acetylation and deacetylation, are catalyzed by histone acetyltransferase (HAT) and histone deacetylase (HDAC), respectively. The Arabidopsis HDACs, HDA6, and HDA19, were reported to function in physiological processes, including embryo development, abiotic stress response, and flowering. In this study, we demonstrated that histone deacetylase inhibitors (HDIs) inhibit primary root elongation and lateral root emergence. In response to HDIs treatment, the PIN1 protein was almost abolished in the root tip. However, the PIN1 gene did not show decreased expression in the presence of HDIs, whereas IAA genes exhibited increases in transcript levels. In contrast, we observed a stable level of gene expression of stress markers (KIN1 and COR15A) and a cell division marker (CYCB1). Taken together, these results suggest that epigenetic regulation may control auxin-mediated root development through the 26S proteasome-mediated degradation of PIN1 protein.

Original language	English
Pages (from-to)	1625-1636
Number of pages	12
Journal	Plant Cell Reports
Volume	32
Issue number	10
DOIs	https://doi.org/10.1007/s00299-013-1474-6
Publication status	Published - 2013 Oct

Bibliographical note

Funding Information:
Acknowledgments We would like to thank to Dr. Kequiang Wu (National Taiwan University) for donating hda19 seeds. This work was supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (to Hojoung Lee, 2012; Grant #2012-112068-3) and by a grant from the National Research Foundation (to Suk-Whan Hong; Grant #2012R1A1A4A01006448).

Keywords

Auxin
Epigenetic
PIN1
Root development
Sodium butyrate (NaB)
Trichostatin A (TSA)

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s00299-013-1474-6

Cite this

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title = "Inhibition of histone deacetylation alters Arabidopsis root growth in response to auxin via PIN1 degradation",

abstract = "Key message: Our results showed the histone deacetylase inhibitors (HDIs) control root development in Arabidopsis via regulation of PIN1 degradation. Epigenetic regulation plays a crucial role in the expression of many genes in response to exogenous or endogenous signals in plants as well as other organisms. One of epigenetic mechanisms is modifications of histone, such as acetylation and deacetylation, are catalyzed by histone acetyltransferase (HAT) and histone deacetylase (HDAC), respectively. The Arabidopsis HDACs, HDA6, and HDA19, were reported to function in physiological processes, including embryo development, abiotic stress response, and flowering. In this study, we demonstrated that histone deacetylase inhibitors (HDIs) inhibit primary root elongation and lateral root emergence. In response to HDIs treatment, the PIN1 protein was almost abolished in the root tip. However, the PIN1 gene did not show decreased expression in the presence of HDIs, whereas IAA genes exhibited increases in transcript levels. In contrast, we observed a stable level of gene expression of stress markers (KIN1 and COR15A) and a cell division marker (CYCB1). Taken together, these results suggest that epigenetic regulation may control auxin-mediated root development through the 26S proteasome-mediated degradation of PIN1 protein.",

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author = "Nguyen, {Hoai Nguyen} and Kim, {Jun Hyeok} and Jeong, {Chan Young} and Hong, {Suk Whan} and Hojoung Lee",

note = "Funding Information: Acknowledgments We would like to thank to Dr. Kequiang Wu (National Taiwan University) for donating hda19 seeds. This work was supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (to Hojoung Lee, 2012; Grant #2012-112068-3) and by a grant from the National Research Foundation (to Suk-Whan Hong; Grant #2012R1A1A4A01006448).",

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AU - Hong, Suk Whan

AU - Lee, Hojoung

N1 - Funding Information: Acknowledgments We would like to thank to Dr. Kequiang Wu (National Taiwan University) for donating hda19 seeds. This work was supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (to Hojoung Lee, 2012; Grant #2012-112068-3) and by a grant from the National Research Foundation (to Suk-Whan Hong; Grant #2012R1A1A4A01006448).

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N2 - Key message: Our results showed the histone deacetylase inhibitors (HDIs) control root development in Arabidopsis via regulation of PIN1 degradation. Epigenetic regulation plays a crucial role in the expression of many genes in response to exogenous or endogenous signals in plants as well as other organisms. One of epigenetic mechanisms is modifications of histone, such as acetylation and deacetylation, are catalyzed by histone acetyltransferase (HAT) and histone deacetylase (HDAC), respectively. The Arabidopsis HDACs, HDA6, and HDA19, were reported to function in physiological processes, including embryo development, abiotic stress response, and flowering. In this study, we demonstrated that histone deacetylase inhibitors (HDIs) inhibit primary root elongation and lateral root emergence. In response to HDIs treatment, the PIN1 protein was almost abolished in the root tip. However, the PIN1 gene did not show decreased expression in the presence of HDIs, whereas IAA genes exhibited increases in transcript levels. In contrast, we observed a stable level of gene expression of stress markers (KIN1 and COR15A) and a cell division marker (CYCB1). Taken together, these results suggest that epigenetic regulation may control auxin-mediated root development through the 26S proteasome-mediated degradation of PIN1 protein.

AB - Key message: Our results showed the histone deacetylase inhibitors (HDIs) control root development in Arabidopsis via regulation of PIN1 degradation. Epigenetic regulation plays a crucial role in the expression of many genes in response to exogenous or endogenous signals in plants as well as other organisms. One of epigenetic mechanisms is modifications of histone, such as acetylation and deacetylation, are catalyzed by histone acetyltransferase (HAT) and histone deacetylase (HDAC), respectively. The Arabidopsis HDACs, HDA6, and HDA19, were reported to function in physiological processes, including embryo development, abiotic stress response, and flowering. In this study, we demonstrated that histone deacetylase inhibitors (HDIs) inhibit primary root elongation and lateral root emergence. In response to HDIs treatment, the PIN1 protein was almost abolished in the root tip. However, the PIN1 gene did not show decreased expression in the presence of HDIs, whereas IAA genes exhibited increases in transcript levels. In contrast, we observed a stable level of gene expression of stress markers (KIN1 and COR15A) and a cell division marker (CYCB1). Taken together, these results suggest that epigenetic regulation may control auxin-mediated root development through the 26S proteasome-mediated degradation of PIN1 protein.

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Inhibition of histone deacetylation alters Arabidopsis root growth in response to auxin via PIN1 degradation

Abstract

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Keywords

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