Inhibition of interleukin-12 production in lipopolysaccharide-activated macrophages by curcumin

Bok Yun Kang; Su Wol Chung; Woon Jae Chung; Suhn Young Im; Seung Yong Hwang; Tae Sung Kim

doi:10.1016/S0014-2999(99)00690-1

Inhibition of interleukin-12 production in lipopolysaccharide-activated macrophages by curcumin

Bok Yun Kang, Su Wol Chung, Woon Jae Chung, Suhn Young Im, Seung Yong Hwang, Tae Sung Kim

Research output: Contribution to journal › Article › peer-review

64 Citations (Scopus)

Abstract

Pharmacological control of interleukin-12 production may be a key therapeutic strategy for modulating immunological diseases dominated by type-1 cytokine responses. In this study we investigated the effects of curcumin (1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6-heptadiene-3,5-dione) on the production of interleukin-12 from mouse macrophages stimulated with lipopolysaccharide. Curcumin potently inhibited the production of interleukin-12 in a dose-dependent manner. The effect of curcumin on interleukin-12 p40 promoter activation was analyzed by transfecting RAW264.7 monocytic cells with p40 promoter/reporter constructs. The repressive effect mapped to a region in the p40 promoter containing a binding site for nuclear factor κB (p40-κB). Furthermore, activation of macrophages by lipopolysaccharide resulted in markedly enhanced binding activity to the κB site, which significantly decreased upon addition of curcumin. These results suggest that curcumin-induced inhibition of interleukin-12 production in macrophages may explain some of the biological effects of curcumin including its anti-inflammatory activity. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	191-195
Number of pages	5
Journal	European Journal of Pharmacology
Volume	384
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-2999(99)00690-1
Publication status	Published - 1999 Nov 19
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank Drs. Giorgio Trinchieri (Wistar Institute, USA), Jae Woon Lee (Chonnam National University, Korea) and Yong-Kyong Choe (KRIBB, Korea) for providing reagents. This work was supported by Grants from the KOSEF (HRC to Tae Sung Kim and Suhn-Young Im).

Keywords

Curcumin
Interleukin-12
Macrophage
Nuclear factor κB

ASJC Scopus subject areas

Pharmacology

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10.1016/S0014-2999(99)00690-1

Cite this

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title = "Inhibition of interleukin-12 production in lipopolysaccharide-activated macrophages by curcumin",

abstract = "Pharmacological control of interleukin-12 production may be a key therapeutic strategy for modulating immunological diseases dominated by type-1 cytokine responses. In this study we investigated the effects of curcumin (1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6-heptadiene-3,5-dione) on the production of interleukin-12 from mouse macrophages stimulated with lipopolysaccharide. Curcumin potently inhibited the production of interleukin-12 in a dose-dependent manner. The effect of curcumin on interleukin-12 p40 promoter activation was analyzed by transfecting RAW264.7 monocytic cells with p40 promoter/reporter constructs. The repressive effect mapped to a region in the p40 promoter containing a binding site for nuclear factor κB (p40-κB). Furthermore, activation of macrophages by lipopolysaccharide resulted in markedly enhanced binding activity to the κB site, which significantly decreased upon addition of curcumin. These results suggest that curcumin-induced inhibition of interleukin-12 production in macrophages may explain some of the biological effects of curcumin including its anti-inflammatory activity. Copyright (C) 1999 Elsevier Science B.V.",

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N1 - Funding Information: We would like to thank Drs. Giorgio Trinchieri (Wistar Institute, USA), Jae Woon Lee (Chonnam National University, Korea) and Yong-Kyong Choe (KRIBB, Korea) for providing reagents. This work was supported by Grants from the KOSEF (HRC to Tae Sung Kim and Suhn-Young Im).

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N2 - Pharmacological control of interleukin-12 production may be a key therapeutic strategy for modulating immunological diseases dominated by type-1 cytokine responses. In this study we investigated the effects of curcumin (1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6-heptadiene-3,5-dione) on the production of interleukin-12 from mouse macrophages stimulated with lipopolysaccharide. Curcumin potently inhibited the production of interleukin-12 in a dose-dependent manner. The effect of curcumin on interleukin-12 p40 promoter activation was analyzed by transfecting RAW264.7 monocytic cells with p40 promoter/reporter constructs. The repressive effect mapped to a region in the p40 promoter containing a binding site for nuclear factor κB (p40-κB). Furthermore, activation of macrophages by lipopolysaccharide resulted in markedly enhanced binding activity to the κB site, which significantly decreased upon addition of curcumin. These results suggest that curcumin-induced inhibition of interleukin-12 production in macrophages may explain some of the biological effects of curcumin including its anti-inflammatory activity. Copyright (C) 1999 Elsevier Science B.V.

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Inhibition of interleukin-12 production in lipopolysaccharide-activated macrophages by curcumin

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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