Inhibition of interleukin-12 production in mouse macrophagesvia decreased nuclear factor-κB DNA binding activity by myricetin, a naturally occurring flavonoid

Bok Yun Kang, Seung Hyun Kim, Daeho Cho, Tae Sung Kim

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing the development and progression of disease in experimental models of autoimmunity. In this study, the effects of myricetin, a naturally occurring flavonoid present in fruits, vegetables and medicinal herbs, on the production of IL-12 were investigated in mouse macrophages stimulated with lipopolysaccharide (LPS). Myricetin significantly inhibited the LPS-induced IL-12 production from both primary macrophages and the RAW264.7 monocytic cell-line in a dose-dependent manner. The effect of myricetin on IL-12 gene promoter activation was analyzed by transfecting RAW264.7 cells with IL-12 gene promoter/luciferase constructs. The repressive effect was mapped to a region in the IL-12 gene promoter containing a binding site for NF-κB. Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF-κB site, which significantly decreased upon addition of myricetin, indicating that myricetin inhibited IL-12 production in LPS-activated macrophagesvia the down-regulation of NF-κB binding activity.

Original languageEnglish
Pages (from-to)274-279
Number of pages6
JournalArchives of pharmacal research
Volume28
Issue number3
DOIs
Publication statusPublished - 2005 Mar
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2005, The Pharmaceutical Society of Korea.

Keywords

  • Interleukin-12
  • Macrophage
  • Myricetin
  • Nuclear factor-κB

ASJC Scopus subject areas

  • Drug Discovery
  • Molecular Medicine
  • Organic Chemistry

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