Inhibition of interleukin-4 production in activated T cells via down-regulation of NF-AT DNA binding activity by apigenin, a flavonoid present in dietary plants

Jin Park, Seung Hyun Kim, Tae Sung Kim

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Apigenin, a flavonoid present in many dietary plants, exhibits anti-allergic activity and inhibits IL-4 production by basophils and mast cells. However, the mechanism by which apigenin suppresses IL-4 production especially in T cells remains unclear. In this study, the effect of apigenin and its underlying mechanism on IL-4 production were investigated in activated T cells. Apigenin significantly inhibited IL-4 production in both EL4 T thymoma cells and primary lymph node cells. Apigenin also inhibited IL-4 gene promoter activity in EL4 cells transiently transfected with IL-4 gene promoter constructs, but this inhibitory effect was impaired in EL4 cells transfected with an IL-4 promoter construct deleted of NF-AT binding sites. In addition, apigenin inhibited NF-AT DNA binding activities, indicating that apigenin may inhibit IL-4 production in T cells via down-regulation of NF-AT DNA binding activity.

Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalImmunology Letters
Volume103
Issue number2
DOIs
Publication statusPublished - 2006 Mar 15

Bibliographical note

Funding Information:
We would like to thank Drs. M. Howard, J.W. Lee and Y.K. Choe for their generous gift of valuable reagents, and Dr. E.P. Cohen for helpful discussions and critical reading of the manuscript. This work was supported by the SRC/ERC program of the KOSEF/MOST (R11-2005-017-02001-0) and the Korea Research Foundation Grant (KRF-2003-005-E00012).

Keywords

  • Allergy
  • Apigenin
  • Interleukin-4
  • NF-AT
  • T cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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