Inhibition of LPS-induced nitric oxide production by transduced Tat-arginine deiminase fusion protein in Raw 264.7 cells

Min Jung Lee; Dae Won Kim; Yeom Pyo Lee; Hoon Jae Jeong; Hye Won Kang; Min Jae Shin; Eun Jeong Sohn; Mi Jin Kim; Sang Ho Jang; Tae Cheon Kang; Moo Ho Won; Bon Hong Min; Sung Woo Cho; Kil Soo Lee; Jinseu Park; Won Sik Eum; Soo Young Choi

doi:10.5483/BMBRep.2009.42.5.286

Inhibition of LPS-induced nitric oxide production by transduced Tat-arginine deiminase fusion protein in Raw 264.7 cells

Min Jung Lee, Dae Won Kim, Yeom Pyo Lee, Hoon Jae Jeong, Hye Won Kang, Min Jae Shin, Eun Jeong Sohn, Mi Jin Kim, Sang Ho Jang, Tae Cheon Kang, Moo Ho Won, Bon Hong Min, Sung Woo Cho, Kil Soo Lee, Jinseu Park, Won Sik Eum, Soo Young Choi

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Arginine deiminase (ADI), an arginine-degrading enzyme, has anti-proliferative and anti-tumor activities and is capable of inhibiting the production of nitric oxide (NO). Modulation of nitric oxide (NO) production is considered a promising approach for the treatment of various diseases including cancer, inflammation and neuronal disorders. In this study, an ADI gene was fused with an HIV-1 Tat peptide in a bacterial expression vector to produce an genetic in-frame Tat-ADI fusion protein. When added exogenously to the culture media, the expressed and purified Tat-ADI fusion proteins were efficiently transduced into macrophage Raw 264.7 cells in a time- and dose-dependent manner. Furthermore, transduced Tat-ADI fusion proteins markedly increased cell viability in cells treated with lipopolysaccharide (LPS). This increase in viability was mediated by an inhibition of NO production. These results suggest that this Tat-ADI fusion protein can be used in protein therapies of NO-related disorders such as cancer, inflammation and neuronal diseases.

Original language	English
Pages (from-to)	286-292
Number of pages	7
Journal	BMB reports
Volume	42
Issue number	5
DOIs	https://doi.org/10.5483/BMBRep.2009.42.5.286
Publication status	Published - 2009 May

Keywords

Arginine deiminase (ADI)
HIV-1 Tat peptide
Inflammation
Nitric oxide synthase
Protein transduction

ASJC Scopus subject areas

Biochemistry
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.5483/BMBRep.2009.42.5.286

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Lee, M. J., Kim, D. W., Lee, Y. P., Jeong, H. J., Kang, H. W., Shin, M. J., Sohn, E. J., Kim, M. J., Jang, S. H., Kang, T. C., Won, M. H., Min, B. H., Cho, S. W., Lee, K. S., Park, J., Eum, W. S., & Choi, S. Y. (2009). Inhibition of LPS-induced nitric oxide production by transduced Tat-arginine deiminase fusion protein in Raw 264.7 cells. BMB reports, 42(5), 286-292. https://doi.org/10.5483/BMBRep.2009.42.5.286

Lee, MJ, Kim, DW, Lee, YP, Jeong, HJ, Kang, HW, Shin, MJ, Sohn, EJ, Kim, MJ, Jang, SH, Kang, TC, Won, MH, Min, BH, Cho, SW, Lee, KS, Park, J, Eum, WS & Choi, SY 2009, 'Inhibition of LPS-induced nitric oxide production by transduced Tat-arginine deiminase fusion protein in Raw 264.7 cells', BMB reports, vol. 42, no. 5, pp. 286-292. https://doi.org/10.5483/BMBRep.2009.42.5.286

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abstract = "Arginine deiminase (ADI), an arginine-degrading enzyme, has anti-proliferative and anti-tumor activities and is capable of inhibiting the production of nitric oxide (NO). Modulation of nitric oxide (NO) production is considered a promising approach for the treatment of various diseases including cancer, inflammation and neuronal disorders. In this study, an ADI gene was fused with an HIV-1 Tat peptide in a bacterial expression vector to produce an genetic in-frame Tat-ADI fusion protein. When added exogenously to the culture media, the expressed and purified Tat-ADI fusion proteins were efficiently transduced into macrophage Raw 264.7 cells in a time- and dose-dependent manner. Furthermore, transduced Tat-ADI fusion proteins markedly increased cell viability in cells treated with lipopolysaccharide (LPS). This increase in viability was mediated by an inhibition of NO production. These results suggest that this Tat-ADI fusion protein can be used in protein therapies of NO-related disorders such as cancer, inflammation and neuronal diseases.",

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AU - Shin, Min Jae

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AU - Kang, Tae Cheon

AU - Won, Moo Ho

AU - Min, Bon Hong

AU - Cho, Sung Woo

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AU - Park, Jinseu

AU - Eum, Won Sik

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Inhibition of LPS-induced nitric oxide production by transduced Tat-arginine deiminase fusion protein in Raw 264.7 cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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