Inhibition of Ras-mediated activator protein 1 activity and cell growth by merlin

Hongtae Kim; Jung Yeon Lim; Young Hoon Kim; Hyun Kim; Sun Hwa Park; Kweon Haeng Lee; Hoon Han; Sin Soo Jeun; Joung H. Lee; Hyoung Kyun Rha

Inhibition of Ras-mediated activator protein 1 activity and cell growth by merlin

Hongtae Kim, Jung Yeon Lim, Young Hoon Kim, Hyun Kim, Sun Hwa Park, Kweon Haeng Lee, Hoon Han, Sin Soo Jeun, Joung H. Lee, Hyoung Kyun Rha

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The neurofibromatosis type2 (NF2) gene encodes an intracellular-associated protein that is referred to as either merlin or schwannomin. Merlin/Schwannomin is a tumor suppressor protein that has been reported to block the Ras-mediated cell proliferation and - anchorage-independent cell growth. However, its inhibitory mechanism is uncertain. In this report, merlin was demonstrated to be effective in suppressing Ras-induced foci-formation and its associated AP-1 activity in NIH3T3cells. In addition, merlin blocked Ras-induced Rb phosphorylation, and inhibited the increase of cyclin D1 levels. It also blocked E2F-1-dependent transcription. These results suggest that merlin inhibits abnormal cell proliferation which is activated via Ras by repressing Rb phosphorylation, blocking the increase of the cyclin D1 protein level, and inhibiting the activation of AP-1- and E2F-1-dependent transcription in NIH3T3 cells.

Original language	English
Pages (from-to)	108-114
Number of pages	7
Journal	Molecules and cells
Volume	14
Issue number	1
Publication status	Published - 2002 Aug

Keywords

AP-1
Cyclin D
Neurofibromatosis Type2 (NF2)
Ras
Retinoblastoma (Rb)

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Cite this

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title = "Inhibition of Ras-mediated activator protein 1 activity and cell growth by merlin",

abstract = "The neurofibromatosis type2 (NF2) gene encodes an intracellular-associated protein that is referred to as either merlin or schwannomin. Merlin/Schwannomin is a tumor suppressor protein that has been reported to block the Ras-mediated cell proliferation and - anchorage-independent cell growth. However, its inhibitory mechanism is uncertain. In this report, merlin was demonstrated to be effective in suppressing Ras-induced foci-formation and its associated AP-1 activity in NIH3T3cells. In addition, merlin blocked Ras-induced Rb phosphorylation, and inhibited the increase of cyclin D1 levels. It also blocked E2F-1-dependent transcription. These results suggest that merlin inhibits abnormal cell proliferation which is activated via Ras by repressing Rb phosphorylation, blocking the increase of the cyclin D1 protein level, and inhibiting the activation of AP-1- and E2F-1-dependent transcription in NIH3T3 cells.",

keywords = "AP-1, Cyclin D, Neurofibromatosis Type2 (NF2), Ras, Retinoblastoma (Rb)",

author = "Hongtae Kim and Lim, {Jung Yeon} and Kim, {Young Hoon} and Hyun Kim and Park, {Sun Hwa} and Lee, {Kweon Haeng} and Hoon Han and Jeun, {Sin Soo} and Lee, {Joung H.} and Rha, {Hyoung Kyun}",

year = "2002",

month = aug,

language = "English",

volume = "14",

pages = "108--114",

journal = "Molecules and cells",

issn = "1016-8478",

publisher = "Korean Society for Molecular and Cellular Biology",

number = "1",

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TY - JOUR

T1 - Inhibition of Ras-mediated activator protein 1 activity and cell growth by merlin

AU - Kim, Hongtae

AU - Lim, Jung Yeon

AU - Kim, Young Hoon

AU - Kim, Hyun

AU - Park, Sun Hwa

AU - Lee, Kweon Haeng

AU - Han, Hoon

AU - Jeun, Sin Soo

AU - Lee, Joung H.

AU - Rha, Hyoung Kyun

PY - 2002/8

Y1 - 2002/8

N2 - The neurofibromatosis type2 (NF2) gene encodes an intracellular-associated protein that is referred to as either merlin or schwannomin. Merlin/Schwannomin is a tumor suppressor protein that has been reported to block the Ras-mediated cell proliferation and - anchorage-independent cell growth. However, its inhibitory mechanism is uncertain. In this report, merlin was demonstrated to be effective in suppressing Ras-induced foci-formation and its associated AP-1 activity in NIH3T3cells. In addition, merlin blocked Ras-induced Rb phosphorylation, and inhibited the increase of cyclin D1 levels. It also blocked E2F-1-dependent transcription. These results suggest that merlin inhibits abnormal cell proliferation which is activated via Ras by repressing Rb phosphorylation, blocking the increase of the cyclin D1 protein level, and inhibiting the activation of AP-1- and E2F-1-dependent transcription in NIH3T3 cells.

AB - The neurofibromatosis type2 (NF2) gene encodes an intracellular-associated protein that is referred to as either merlin or schwannomin. Merlin/Schwannomin is a tumor suppressor protein that has been reported to block the Ras-mediated cell proliferation and - anchorage-independent cell growth. However, its inhibitory mechanism is uncertain. In this report, merlin was demonstrated to be effective in suppressing Ras-induced foci-formation and its associated AP-1 activity in NIH3T3cells. In addition, merlin blocked Ras-induced Rb phosphorylation, and inhibited the increase of cyclin D1 levels. It also blocked E2F-1-dependent transcription. These results suggest that merlin inhibits abnormal cell proliferation which is activated via Ras by repressing Rb phosphorylation, blocking the increase of the cyclin D1 protein level, and inhibiting the activation of AP-1- and E2F-1-dependent transcription in NIH3T3 cells.

KW - AP-1

KW - Cyclin D

KW - Neurofibromatosis Type2 (NF2)

KW - Ras

KW - Retinoblastoma (Rb)

UR - http://www.scopus.com/inward/record.url?scp=18744428417&partnerID=8YFLogxK

M3 - Article

C2 - 12243339

AN - SCOPUS:18744428417

SN - 1016-8478

VL - 14

SP - 108

EP - 114

JO - Molecules and cells

JF - Molecules and cells

IS - 1

ER -

Inhibition of Ras-mediated activator protein 1 activity and cell growth by merlin

Abstract

Keywords

ASJC Scopus subject areas

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