Inhibition of receptor internalization attenuates the TNFα-induced ROS generation in non-phagocytic cells

Chang Hoon Woo; Tae Hee Kim; Jung A. Choi; Ho Cheol Ryu; Jung Eun Lee; Hye Jin You; Yun Soo Bae; Jae Hong Kim

doi:10.1016/j.bbrc.2006.10.154

Inhibition of receptor internalization attenuates the TNFα-induced ROS generation in non-phagocytic cells

Chang Hoon Woo, Tae Hee Kim, Jung A. Choi, Ho Cheol Ryu, Jung Eun Lee, Hye Jin You, Yun Soo Bae, Jae Hong Kim

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) are important regulatory molecules implicated in the signaling cascade triggered by tumor necrosis factor (TNF)α, although the events through which TNFα induces ROS generation are not well characterized. Here, we report that TNFα-induced ROS production was blocked by pretreatment with internalization inhibitor monodansyl cadaverine (MDC). Similarly, a transient expression of a GTP-binding and hydrolysis-defective dynamin mutant (dynamin^K44A) that had been shown to be defective in internalization significantly attenuated the TNFα-induced intracellular ROS production. Importantly, the inhibition of receptor internalization suppressed TNFα signaling to mitogen-activated protein kinases (MAPKs) stimulation. Together, our results suggest that receptor internalization is somehow necessary for the TNFα-induced ROS generation and subsequent intracellular downstream signaling in non-phagocytes.

Original language	English
Pages (from-to)	972-978
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	351
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2006.10.154
Publication status	Published - 2006 Dec 29

Bibliographical note

Funding Information:
This work was supported by the Frontier 21 Programs (Proteomics), the BioDiscovery Research Program of the Korea Ministry of Science and Technology, and the SRC program (Aging and Apoptosis Research Center) (2002) of the Korea Science and Engineering Foundation (KOSEF).

Keywords

Non-phagocytes
Nox
ROS
Receptor internalization
Signal transduction
TNFα

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2006.10.154

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title = "Inhibition of receptor internalization attenuates the TNFα-induced ROS generation in non-phagocytic cells",

abstract = "Reactive oxygen species (ROS) are important regulatory molecules implicated in the signaling cascade triggered by tumor necrosis factor (TNF)α, although the events through which TNFα induces ROS generation are not well characterized. Here, we report that TNFα-induced ROS production was blocked by pretreatment with internalization inhibitor monodansyl cadaverine (MDC). Similarly, a transient expression of a GTP-binding and hydrolysis-defective dynamin mutant (dynaminK44A) that had been shown to be defective in internalization significantly attenuated the TNFα-induced intracellular ROS production. Importantly, the inhibition of receptor internalization suppressed TNFα signaling to mitogen-activated protein kinases (MAPKs) stimulation. Together, our results suggest that receptor internalization is somehow necessary for the TNFα-induced ROS generation and subsequent intracellular downstream signaling in non-phagocytes.",

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author = "Woo, {Chang Hoon} and Kim, {Tae Hee} and Choi, {Jung A.} and Ryu, {Ho Cheol} and Lee, {Jung Eun} and You, {Hye Jin} and Bae, {Yun Soo} and Kim, {Jae Hong}",

note = "Funding Information: This work was supported by the Frontier 21 Programs (Proteomics), the BioDiscovery Research Program of the Korea Ministry of Science and Technology, and the SRC program (Aging and Apoptosis Research Center) (2002) of the Korea Science and Engineering Foundation (KOSEF).",

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doi = "10.1016/j.bbrc.2006.10.154",

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AU - Woo, Chang Hoon

AU - Kim, Tae Hee

AU - Choi, Jung A.

AU - Ryu, Ho Cheol

AU - Lee, Jung Eun

AU - You, Hye Jin

AU - Bae, Yun Soo

AU - Kim, Jae Hong

N1 - Funding Information: This work was supported by the Frontier 21 Programs (Proteomics), the BioDiscovery Research Program of the Korea Ministry of Science and Technology, and the SRC program (Aging and Apoptosis Research Center) (2002) of the Korea Science and Engineering Foundation (KOSEF).

PY - 2006/12/29

Y1 - 2006/12/29

N2 - Reactive oxygen species (ROS) are important regulatory molecules implicated in the signaling cascade triggered by tumor necrosis factor (TNF)α, although the events through which TNFα induces ROS generation are not well characterized. Here, we report that TNFα-induced ROS production was blocked by pretreatment with internalization inhibitor monodansyl cadaverine (MDC). Similarly, a transient expression of a GTP-binding and hydrolysis-defective dynamin mutant (dynaminK44A) that had been shown to be defective in internalization significantly attenuated the TNFα-induced intracellular ROS production. Importantly, the inhibition of receptor internalization suppressed TNFα signaling to mitogen-activated protein kinases (MAPKs) stimulation. Together, our results suggest that receptor internalization is somehow necessary for the TNFα-induced ROS generation and subsequent intracellular downstream signaling in non-phagocytes.

AB - Reactive oxygen species (ROS) are important regulatory molecules implicated in the signaling cascade triggered by tumor necrosis factor (TNF)α, although the events through which TNFα induces ROS generation are not well characterized. Here, we report that TNFα-induced ROS production was blocked by pretreatment with internalization inhibitor monodansyl cadaverine (MDC). Similarly, a transient expression of a GTP-binding and hydrolysis-defective dynamin mutant (dynaminK44A) that had been shown to be defective in internalization significantly attenuated the TNFα-induced intracellular ROS production. Importantly, the inhibition of receptor internalization suppressed TNFα signaling to mitogen-activated protein kinases (MAPKs) stimulation. Together, our results suggest that receptor internalization is somehow necessary for the TNFα-induced ROS generation and subsequent intracellular downstream signaling in non-phagocytes.

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KW - Signal transduction

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Inhibition of receptor internalization attenuates the TNFα-induced ROS generation in non-phagocytic cells

Abstract

Bibliographical note

Keywords

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