Inhibition of Staphylococcus aureus by antimicrobial biofilms formed by competitive exclusion microorganisms on stainless steel

Hyeri Son; Sunhyung Park; Larry R. Beuchat; Hoikyung Kim; Jee Hoon Ryu

doi:10.1016/j.ijfoodmicro.2016.09.007

Inhibition of Staphylococcus aureus by antimicrobial biofilms formed by competitive exclusion microorganisms on stainless steel

Hyeri Son, Sunhyung Park, Larry R. Beuchat, Hoikyung Kim, Jee Hoon Ryu

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The goal of this study was to develop a desiccation resistant antimicrobial surface using biofilm of competitive exclusion (CE) microorganism inhibitory to Staphylococcus aureus. We isolated 161 microorganisms from soils, foods, and food-contact surfaces that are inhibitory to S. aureus. Among them, three CE microorganisms (Streptomyces spororaveus strain Gaeunsan-18, Bacillus safensis strain Chamnamu-sup 5–25, and Pseudomonas azotoformans strain Lettuce-9) exhibiting strong antibacterial activity and high growth rates were selected for evaluation. These isolates formed biofilms within 24 h on stainless steel coupons (SSCs) immersed in Bennet's broth and tryptic soy broth at 25 °C. Cells in these biofilms showed significantly (P ≤ 0.05) enhanced resistance to a desiccation (43% relative humidity [RH]) compared to those attached to SSCs but not in biofilms. The antimicrobial activities of biofilms formed by these isolates on SSCs against S. aureus at 25 °C and 43% RH were determined. Compared to SSCs lacking biofilms formed by CE microorganisms, populations of S. aureus on SSCs harboring CE biofilms were significantly lower (P ≤ 0.05). Results indicate that persistent antimicrobial activity against S. aureus on stainless steel surfaces can be achieved by the presence of biofilms of CE microorganisms. This information will be useful when developing strategies to improve the microbiological safety of foods during storage, processing, and distribution by facilitating the development of effective antimicrobial food-contact surfaces.

Original language	English
Pages (from-to)	165-171
Number of pages	7
Journal	International Journal of Food Microbiology
Volume	238
DOIs	https://doi.org/10.1016/j.ijfoodmicro.2016.09.007
Publication status	Published - 2016 Dec 5

Bibliographical note

Funding Information:
This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) ( NRF-2013R1A2A2A01068475 ) and by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries , Republic of Korea (No. 316011-05-1-HD060 ).

Publisher Copyright:
© 2016

Keywords

Antimicrobial surface
Biofilm
Competitive-exclusion microorganisms
Stainless steel
Staphylococcus aureus

ASJC Scopus subject areas

Food Science
Microbiology

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10.1016/j.ijfoodmicro.2016.09.007

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title = "Inhibition of Staphylococcus aureus by antimicrobial biofilms formed by competitive exclusion microorganisms on stainless steel",

abstract = "The goal of this study was to develop a desiccation resistant antimicrobial surface using biofilm of competitive exclusion (CE) microorganism inhibitory to Staphylococcus aureus. We isolated 161 microorganisms from soils, foods, and food-contact surfaces that are inhibitory to S. aureus. Among them, three CE microorganisms (Streptomyces spororaveus strain Gaeunsan-18, Bacillus safensis strain Chamnamu-sup 5–25, and Pseudomonas azotoformans strain Lettuce-9) exhibiting strong antibacterial activity and high growth rates were selected for evaluation. These isolates formed biofilms within 24 h on stainless steel coupons (SSCs) immersed in Bennet's broth and tryptic soy broth at 25 °C. Cells in these biofilms showed significantly (P ≤ 0.05) enhanced resistance to a desiccation (43% relative humidity [RH]) compared to those attached to SSCs but not in biofilms. The antimicrobial activities of biofilms formed by these isolates on SSCs against S. aureus at 25 °C and 43% RH were determined. Compared to SSCs lacking biofilms formed by CE microorganisms, populations of S. aureus on SSCs harboring CE biofilms were significantly lower (P ≤ 0.05). Results indicate that persistent antimicrobial activity against S. aureus on stainless steel surfaces can be achieved by the presence of biofilms of CE microorganisms. This information will be useful when developing strategies to improve the microbiological safety of foods during storage, processing, and distribution by facilitating the development of effective antimicrobial food-contact surfaces.",

keywords = "Antimicrobial surface, Biofilm, Competitive-exclusion microorganisms, Stainless steel, Staphylococcus aureus",

author = "Hyeri Son and Sunhyung Park and Beuchat, {Larry R.} and Hoikyung Kim and Ryu, {Jee Hoon}",

note = "Funding Information: This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) ( NRF-2013R1A2A2A01068475 ) and by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries , Republic of Korea (No. 316011-05-1-HD060 ). Publisher Copyright: {\textcopyright} 2016",

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AU - Son, Hyeri

AU - Park, Sunhyung

AU - Beuchat, Larry R.

AU - Kim, Hoikyung

AU - Ryu, Jee Hoon

N1 - Funding Information: This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) ( NRF-2013R1A2A2A01068475 ) and by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries , Republic of Korea (No. 316011-05-1-HD060 ). Publisher Copyright: © 2016

PY - 2016/12/5

Y1 - 2016/12/5

N2 - The goal of this study was to develop a desiccation resistant antimicrobial surface using biofilm of competitive exclusion (CE) microorganism inhibitory to Staphylococcus aureus. We isolated 161 microorganisms from soils, foods, and food-contact surfaces that are inhibitory to S. aureus. Among them, three CE microorganisms (Streptomyces spororaveus strain Gaeunsan-18, Bacillus safensis strain Chamnamu-sup 5–25, and Pseudomonas azotoformans strain Lettuce-9) exhibiting strong antibacterial activity and high growth rates were selected for evaluation. These isolates formed biofilms within 24 h on stainless steel coupons (SSCs) immersed in Bennet's broth and tryptic soy broth at 25 °C. Cells in these biofilms showed significantly (P ≤ 0.05) enhanced resistance to a desiccation (43% relative humidity [RH]) compared to those attached to SSCs but not in biofilms. The antimicrobial activities of biofilms formed by these isolates on SSCs against S. aureus at 25 °C and 43% RH were determined. Compared to SSCs lacking biofilms formed by CE microorganisms, populations of S. aureus on SSCs harboring CE biofilms were significantly lower (P ≤ 0.05). Results indicate that persistent antimicrobial activity against S. aureus on stainless steel surfaces can be achieved by the presence of biofilms of CE microorganisms. This information will be useful when developing strategies to improve the microbiological safety of foods during storage, processing, and distribution by facilitating the development of effective antimicrobial food-contact surfaces.

AB - The goal of this study was to develop a desiccation resistant antimicrobial surface using biofilm of competitive exclusion (CE) microorganism inhibitory to Staphylococcus aureus. We isolated 161 microorganisms from soils, foods, and food-contact surfaces that are inhibitory to S. aureus. Among them, three CE microorganisms (Streptomyces spororaveus strain Gaeunsan-18, Bacillus safensis strain Chamnamu-sup 5–25, and Pseudomonas azotoformans strain Lettuce-9) exhibiting strong antibacterial activity and high growth rates were selected for evaluation. These isolates formed biofilms within 24 h on stainless steel coupons (SSCs) immersed in Bennet's broth and tryptic soy broth at 25 °C. Cells in these biofilms showed significantly (P ≤ 0.05) enhanced resistance to a desiccation (43% relative humidity [RH]) compared to those attached to SSCs but not in biofilms. The antimicrobial activities of biofilms formed by these isolates on SSCs against S. aureus at 25 °C and 43% RH were determined. Compared to SSCs lacking biofilms formed by CE microorganisms, populations of S. aureus on SSCs harboring CE biofilms were significantly lower (P ≤ 0.05). Results indicate that persistent antimicrobial activity against S. aureus on stainless steel surfaces can be achieved by the presence of biofilms of CE microorganisms. This information will be useful when developing strategies to improve the microbiological safety of foods during storage, processing, and distribution by facilitating the development of effective antimicrobial food-contact surfaces.

KW - Antimicrobial surface

KW - Biofilm

KW - Competitive-exclusion microorganisms

KW - Stainless steel

KW - Staphylococcus aureus

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SN - 0168-1605

VL - 238

SP - 165

EP - 171

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

ER -

Inhibition of Staphylococcus aureus by antimicrobial biofilms formed by competitive exclusion microorganisms on stainless steel

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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