Inhibitory activity of hinokitiol against biofilm formation in fluconazole-resistant Candida species

Dae Jin Kim, Min Woo Lee, Jeong Su Choi, Seung Gwan Lee, Jee Yoon Park, Suhng Wook Kim

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


The aim of this study was to investigate the ability of hinokitiol to inhibit the formation of Candida biofilms. Biofilm inhibition was evaluated by quantification of the biofilm metabolic activity with XTT assay. Hinokitiol efficiently prevented biofilm formation in both fluconazole-susceptible and fluconazole-resistant strains of Candida species. We determined the expression levels of specific genes previously implicated in biofilm development of C. albicans cells by realtime RT-PCR. The expression levels of genes associated with adhesion process, HWP1 and ALS3, were downregulated by hinokitiol. Transcript levels of UME6 and HGC1, responsible for long-Term hyphal maintenance, were also decreased by hinokitiol. The expression level of CYR1, which encodes the component of signaling pathway of hyphal formation-cAMP-PKA was suppressed by hinokitiol. Its upstream general regulator RAS1 was also suppressed by hinokitiol. These results indicate that hinokitiol may have therapeutic potential in the treatment and prevention of biofilm-Associated Candida infections.

Original languageEnglish
Article numbere0171244
JournalPloS one
Issue number2
Publication statusPublished - 2017 Feb

Bibliographical note

Funding Information:
This research was supported by a grant from Korea University (K1508301)

Publisher Copyright:
© 2017 Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • General Agricultural and Biological Sciences
  • General


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